Expression and clinical significance of musashi RNA-binding protein 1 in prostate cancer
Objective To investigate the expression and regulatory function of RNA binding pro-tein musashi RNA-binding protein 1(MSI1)in prostate cancer(PCa).Methods The differential analy-sis,survival analysis and clinical correlation analysis of MSI1 gene were performed based on the cancer ge-nome atlas(TCGA)database.By knocking down the target gene in DU 145 cells,the effect of MSI1 on the proliferation,migration and invasion of prostate cancer cells DU 145 was explored,and the t test was used for comparison between groups.The target gene MSI1 was knocked down at two levels,and its effects on the viability,proliferation and invasion of prostate cancer cells were investigated.Results The expression level of MSI1 in prostate cancer was significantly higher than that in normal prostate tissue,and the differ-ence was statistically significant(7.75±1.01 vs.4.56±0.54,t=4.82,P＜0.01).The results of sur-vival analysis showed that the disease-free survival of the low-expression MSI1 group was significantly longer than that of the high-expression group[hazard ratio(HR)=1.71(1.15-2.53),P＜0.01].There was no statistically significant difference between the expression levels of MSI1 in T1+T2 stage and T3+T4 stage(t=0.38,P＞0.05).MSI expression level[HR:1.47(1.02-2.10),P＜0.05],T stage[HR:1.93(1.02-3.60),P＜0.05]and Gleason score[HR:3.17(1.59-6.30),P＜0.01]could be used as prognos-tic factors for prostate cancer.Compared with normal prostate samples,the expression level of MSI1 in prima-ry prostate cancer samples was significantly increased(P＜0.01),and the progression-free survival time of patients with low MSI1 expression was significantly longer than that of patients with high MSI1 expression(P＜0.05).There was no significant difference in the expression level of MSI1 with T stage,N stage and Gl-eason score in prostate cancer patients(P＞0.05),while T stage and Gleason score including MSI1 could be used as risk factors for prostate cancer(P＜0.05).After MSI1 gene knockdown,the viability,proliferation and invasion ability of prostate cancer cells were measured.cell counting kit-8(CCK-8)results:on day 4,the absorbance value Optical density of Ctrl DU145 cells at a wavelength of 450 nm was 0.929±0.085,while the absorbance(A)values of shMSI1#1 and shMSI1#2 DU145 experimental groups on day 4 were 0.687±0.005 and 0.711±0.018 respectively,the different between them was significant(t=42.21,44.19,P＜0.01).In DU145,the wound healing area of the ctrl group was significantly higher than that of the si-MSI1#1 and si-MSI1#2 groups,and the difference between the groups was statistically significant(P＜0.01).Furthermore,the number of PC3 migrated cells in the ctrl group,si-MSI1#1 and si-MSI1#2 groups were 790.9±80.7,403.3±49.9 and 504.6±117.3 respectively.The difference is statistically significant(t=21.80,13.55,P＜0.01).Conclusion The expression of MSI1 is high in prostate cancer tissues and is related to the poor prognosis of prostate cancer patients.MSI1 gene may play an important role in the proliferation and invasion of prostate cancer.MSI1 is expected to be a potential biomarker for the diagnosis of prostate cancer and a potential target for drug treatment and prognosis prediction.
Prostate cancerRNA-binding proteinMusashi RNA-binding protein 1Data base
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