Effect of expression of Yes-associated protein 1 on proliferation and invasion in esophageal cancer cells
Objective To explore the effect of expression of Yes-associated protein 1(YAP1)on proliferation and invasion of esophageal cancer cells.Methods The transfection efficacy of small interfer-ing RNA(siRNA)YAP1-1,2,3 was detected by real time fluorescence quantitative polymerase chain re-action(Real-time PCR)and Western blotting.Cells were randomly divided into control and siRNA groups.Cell viability was measured by methyl thiazolyl tetrazolium(MTT)assay.Cell clone ability was detected by cell clone formation test.Cell apoptosis was detected by flow cytometry.Cell invasion ability was detected by Transwell method.The expression of B-lymphomatoma-2(bcl-2),bcl-2 related protein X(bax),p53,matrix metalloproteinase-2(MMP-2),MMP-9,c-Fos,c-Jun,and p-c-Jun N-terminal kinase(JNK)was de-tected by Western blotting.Inter group differences were analyzed by t-test.Results The expression of YAP1 mRNA in siRNA YAP1-1,2,3 groups was lower than that in the control group(0.75±0.04,0.45±0.01,0.31±0.01 vs.1.00±0.08,t=5.621,6.324,6.897,P＜0.05).The expression level of YAP1 in siRNA YAP1-1,2,3 groups was lower than that in the control group(0.62±0.03,0.34±0.01,0.20±0.01 vs.0.94±0.07,t=5.904,6.561,6.438,P＜0.05).Therefore,siRNA-YAP1-3 was select-ed as the subsequent experimental research object in this experiment.Cell viability(0.35±0.01 vs.0.58±0.04,t=5.761,P＜0.05),cell clone number[(35.67±3.54)cells vs.(189.65±12.64)cells,t=6.549,P＜0.05],cell invasion number[(34.76±2.45)cells vs.(164.38±10.41)cells,t=6.341,P＜0.05],the expression of bcl-2(0.89±0.07 vs.0.32±0.02,t=5.967,P＜0.05),MMP-2(0.92±0.07 vs.0.37±0.02,t=6.214,P＜0.05)and MMP-9(1.09±0.08 vs.0.38±0.02,t=6.287,P＜0.05)in the siRNA group were significantly reduced as compared with those in the control group.Cell early apoptosis rate[(21.57±1.68)％vs.(2.47±0.02)％,t=5.689,P＜0.05],late apoptosis rate[(17.42±1.36)％vs.(3.26±0.02)％,t=6.236,P＜0.05],the expression of bax(0.25±0.01 vs.0.94±0.06,t=6.325,P＜0.05),p53(0.33±0.02 vs.0.87±0.07,t=6.761,P＜0.05),c-Jun(0.36±0.02 vs.1.05±0.08,t=6.698,P＜0.05),c-Fos(0.35±0.02 vs.0.62±0.04,t=5.749,P＜0.05)and p-JNK(0.26±0.02 vs.1.02±0.08,t=6.325,P＜0.05)in the siRNA group were sig-nificantly increased as compared with those in the control group.Conclusion Down-regulation of the YAP1 expression might inhibit the proliferation and invasion of esophageal cancer cell EC109 by activating the JNK signaling pathway.
Esophageal cancerYes-associated protein 1C-Jun N-terminal kinaseProlif-erationInvasion
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