Effect of B cell translocation gene 1 on proliferation of lymphoma B cells
Objective To investigate the expression of B-cell translocation gene 1(BTG1)in dif-fuse large B-cell lymphoma and its effect on the proliferation and apoptosis of SU-DHL-2 cells by inducing autophagy.Methods The expression levels of BTG1 mRNA and protein in diffuse large B-cell lymphoma cell lines U2932,OCI-LY-10,SU-DHL-2 and B lymphoblast-9 were detected by real-time quantitative re-verse transcription polymerase chain reaction(RT-qPCR)and Western blotting,respectively.SU-DHL-2 cells were randomly divided into control group,empty carrier group,BTG1 overexpression group,3-MA group and BTG1 overexpression+3-MA group.Cell proliferation activity was detected by cell counting kit-8(CCK-8)method,cell apoptosis rate was detected by flow cytometry,and protein expression levels of LC3 Ⅱ,Atg5 and Beclin1 were detected by Western blotting.One way ANOVA was used for multi-group comparison,and LSD-t test was used for pairwise comparison between groups.Results The mRNA ex-pression of BTG1 in U2932,OCI-LY-10 and SU-DHL-2 cells(0.85±0.06,0.54±0.06,0.53±0.05)was lower than that in IM-9 cells(1.22±0.16,t=3.750,6.893,7.129,and P＜0.05).Protein ex-pression levels in U2932,OCI-LY-10 and SU-DHL-2 cells(0.70±0.08,0.54±0.06,0.24±0.04)were all lower than those in IM-9 cells(0.87±0.06,t=2.944,6.736,15.132,all P＜0.05).The cell proliferation activity of BTG1 overexpression group[(74.80±1.24)％]was significantly lower than that of control group(100.00％,t=35.200,P＜0.05),and empty carrier group[(98.35±1.02)％,t=25.404,P＜0.05].The apoptosis rate of BTG1 overexpression group[(14.74±0.93)％]was signifi-cantly higher than that of control group[(6.28±0.31)％,t=14.948,P＜0.05],and empty carrier group[(8.53±0.55)％,t=9.955,P＜0.05].The mRNA expression of BTG1 in overexpression group(2.33±0.08)was significantly higher than that in control group(1.03±0.06,t=22.517,P＜0.05),and empty carrier group(1.01±0.07,t=21.508,P＜0.05).Protein expression level of BTG1 in overexpres-sion group(0.88±0.07)was significantly higher than that of control group(0.56±0.07,t=5.599,P＜0.05),and empty carrier group(0.61±0.06,t=5.072,P＜0.05).The cell proliferation activity in BTG1 overexpression+3-MA group[(84.21±3.26)％]was significantly higher than that in BTG1 overexpression group[(73.42±3.48)％,t=3.919,P＜0.05],and the cell apoptosis rate[(18.40±2.26)％]was sig-nificantly lower than that in BTG1 overexpression group[(26.79±2.80)％,t=4.039,P＜0.05].The protein expression levels of LC3 Ⅱ,Beclin1 and Atg5 in BTG1 overexpression+3-MA group(0.56±0.05,0.72±0.07,0.49±0.05)were significantly lower than those in BTG1 overexpression group(0.71±0.06,0.89±0.05,0.68±0.05,t=3.326,3.423,4.654,all P＜0.05).Conclusion Overexpression of BTG1 plays an anti-diffuse large B-cell lymphoma role by inducing autophagy.
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