Effects of LINC00963 regulating microRNA-506-3p/laminin gamma 1 axis on proliferation,migra-tion and invasion of gastric cancer cells
Objective To investigate the effects of LINC00963 on the proliferation,migration and invasion of gastric cancer(GC)cells by regulating microRNA(miRNA,miR)-506-3p/laminin gamma 1(LAMC1)axis.Methods Totally,98 GC and adjacent tissue samples from Cangzhou Central Hospital,Fengfeng General Hospital of North China Medical and Health Group and Langfang People's Hospital from December 2022 to December 2023 were selected as the study objects,and the expression levels of LINC00963 and miR-506-3p were analyzed by fluorescence quantitative polymerase chain reaction(PCR).Lentivirus was used to establish LINC00963 knockdown(KD)cell lines and miR-506-3p overexpression cell lines,which were long non-coding RNA(lncRNA)control group,LINC00963 KD group,miRNA con-trol group and miR-506-3p group,respectively.Cell counting reagent was used to determine cell prolifera-tion ability.The migration and invasion ability of the cells was analyzed by scratch assay and Transwell as-say.Bioinformatics and dual luciferase reporter gene were used to analyze the relationship between LINC00963 and miR-506-3p.Target genes of miR-506-3p were analyzed,and the effects of LINC00963 and miR-506-3p on the expression of target genes were analyzed by Western blotting.Independent samples t test was used to compare measurement data between groups.Results The expression level of LINC00963 in adjacent tissues(1.03±0.17)was significantly lower than that in GC tissues(1.65±0.15,t=27.210,P＜0.05).The expression level of miR-506-3p in adjacent tissues(0.92±0.20)was significant-ly higher than that in GC tissues(0.60±0.14,t=12.760,P＜0.05).The absorbance value,migration number and invasion number of lncRNA control group[(1.71±0.09),(87.67±5.47)cells and(117.67±12.27)cells]were significantly increased as compared with those of LINC00963 KD group[(1.08±0.18),(54.67±5.28)cells,(86.50±6.95)cells,t=7.556,10.640,5.412,P＜0.05].The absorbance value,migration number and invasion number of cells in miRNA control group[(1.87±0.07),(81.67±9.63)cells,(113.17±8.87)cells]were significantly greater than those in miR-506-3p group[(1.13±0.09),(60.67±8.04)cells,(85.50±5.13)cells,t=16.070,5.596,6.605,P＜0.05].The LAMC1 protein expression level in lncRNA control group(0.99±0.11)was significantly higher than that in L1NC00963 KD group(0.53±0.07,t=8.552,P＜0.05).The LAMC1 protein ex-pression level in miRNA control group(1.08±0.08)was significantly higher than that in miR-506-3p group(0.48±0.09,t=12.540,P＜0.05).Conclusion LINC00963 is highly expressed in GC tissues,and can promote the proliferation,migration and invasion of GC cells by regulating the miR-506-3p/LAMC1 axis.
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