Mechanism of NOC2L protein suppressing the migration of prostate cells in epithelial-mesenchymal transition
Objective To investigate the effect of NOC2L on the migration of prostate cancer cells,and to systematically analyze the molecular mechanism of the contrary effect of NOC2L in the occur-rence of epithelial-mesenchymal transition(EMT).Methods The cancer genome atlas(TCGA),Ciopro-tal and HPA databases were used to analyze the differential expression of NOC2L.Transwell,cell counting kit-8(CCK-8)and plate cloning assays were used to verify the molecular functions.Western blotting and polymerase chain reaction(PCR)were used to explore the molecular mechanism.Results TCGA:Com-pared to normal prostate tissue,the expression of NOC2L gene was relatively higher in prostate cancer tis-sue(prostate cancer tissue vs.normal prostate tissue:5.83 vs.5.43,P＞0.05);HPA:NOC2L content was higher in high-grade prostate cancer(relative chromatin staining score:high-grade 6.828±0.669 vs.3.909±0.222,t=4.15,P＜0.05).NOC2L knockdown inhibited cell migration and proliferation,and the number of clones formed after NOC2L knockdown was less than that in control group(PC3:542.00±35.23 vs.254.00±11.55,t=7.77,P＜0.05;RM1:1406.00±15.34 vs.754.00±75.92,t=8.41,P＜0.05);The absorbance of CCK8 culture after NOC2L knockdown was lower than that of control group(PC3:1.657±0.028 vs.0.972±0.014,t=21.86,P＜0.05;RM1:1.643±0.007 vs.2.931±0.105,t=12.20,P＜0.05).Cell migration was significantly lower after NOC2L knockdown than that of control group(PC3:704.00±26.12 vs.152.80±6.08,t=20.55,P＜0.05;RM1:704.20±37.56 vs.208.40±7.26,t=12.96,P＜0.05).Overexpression of ZEB1 inhibited the mRNA expression of NOC2L(PC3:1.001±0.028 vs.0.377±0.018,t=18.62,P＜0.05;RM1:1.000±0.012 vs.0.529±0.011,t=28.42,P＜0.05),and NOC2L reversed function and inhibited cell migration when ZEB1 expression was elevated.Compared with ZEB1 group,after overexpression of NOC2L,the number of migrating cells decreased(PC3:899.80±31.17 vs.438.00±12.55,t=13.74,P＜0.05;RM1:986.80±65.83 vs.466.40±9.67,t=7.82,P＜0.05).Conclusion NOC2L promotes cell prolifera-tion and migration.During the occurrence of EMT,NOC2L inhibits the function of CDH1 and inhibits cell migration by inhibiting VIM and CDH2.
Prostate cancerEpithelial-mesenchymal transitionZinc finger E-box binding protein 1
NETL
NSTL
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