Changes of serum glycolytic metabolites and their effects on T lymphocyte function in patients with sepsis
Objective To investigate the changes of serum glycolytic metabolites and their effects on T lymphocyte function in patients with sepsis.Methods A total of 54 patients with sepsis admitted to our hospital from August 2022 to December 2023 and 54 healthy volunteers during the same period were se-lected as the study objects.Lactic acid detection kit(colorimetric method)was used to detect the content of serum lactic acid in the two groups.The expression of tumor necrosis factor-α(TNF-α),interleukin(IL)-1β and IL-10 in serum of the two groups was detected by enzyme-linked immunosorbent assay(ELISA).H9 human lymphoid cell lines were divided into lactic acid treated group and untreated group.8 mmol/L of lactic acid was added to the lactic acid treated group,and equal volume of normal saline was added to the the untreated group,and H9 cells were incubated for 24 h.Cell proliferation assay kit(MTT method)was used to detect proliferation activity.The proportion of T lymphocyte subsets was detected by flow cytometry.The mRNA expression of TNF-α,IL-1β and IL-10 was analyzed by fluorescence quantita-tive polymerase chain reaction(PCR).Results The lactic acid level in sepsis patients[(5.51±1.14)mmol/L]was significantly higher than that in control group[(2.01±0.66)mmol/L,t=18.74,P＜0.05].The levels of TNF-α[(240.38±58.50)pg/ml]and IL-1 β[(117.39±16.35)pg/ml]in sepsis patients were significantly higher than those of control patients[(78.52±14.87),(35.89±8.34)pg/ml],whereas the IL-10[(35.43±8.58)pg/ml]level significantly lower than the control group[(99.21±14.76)pg/ml,t=18.96,31.40,26.42,P＜0.05].The absorbance in lactic acid treated group(0.74±0.04)was significantly lower than in untreated group(1.24±0.08,t=13.56,P＜0.05).The percentage of CD8+T lymphocytes[(27.07±1.18)％]in lactic acid treated group was significantly high-er than that in untreated group[(23.70±0.98)％].The percentage of CD4+T lymphocytes[(29.52±0.74)％]and CD4+/CD8+T ratio(1.09±0.04)in lactic acid treated group were significantly lower than those in untreated group[(34.92±2.81)％,1.47-0.12,t=5.40,4.55,7.24,P＜0.05].The mRNA expression of TNF-α(1.18±0.08)and IL-1β(1.32±0.07)in lactic acid treated group was significantly higher than that in untreated group(0.47±0.04,0.59±0.06),whereas IL-10(0.56±0.06)was signif-icantly lower than that of untreated group(1.05±0.09,t=19.90,18.51,11.68,P＜0.05).Conclu-sion The serum levels of lactic acid increased significantly in patients with sepsis.The increase of lactic acid can regulate the level of inflammatory factors by inhibiting the proliferation and differentiation of T lym-phocytes,and ultimately lead to impaired cellular immune function.
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