Expression of long non-coding RNA OIP5-AS1 in colorectal cancer and its relationship with cell proliferation and metastasis
Objective To investigate the expression of long non-coding RNA(lncRNA)OIP5-AS1 in colorectal cancer and its relationship with cell proliferation and metastasis.Methods Totally,51 patients with colorectal cancer who received radical resection in Zhumadian Central Hospital and the First Affiliated Hospital of Xinxiang Medical College,Henan Province from February 2023 to February 2024 were selected as the study objects.The mRNA expression of lncRNA OIP5-AS1 in colorectal cancer tissues and adjacent tissues was detected by fluorescence quantitative polymerase chain reaction(PCR).The short hairpin RNA empty vector(shRNA-control)and OIP5-AS1 knockdown plasmid(shRNA-OIP5-AS1)were transfected into HCT116 colorectal cancer cells,and the proliferative activity of shRNA-control and shRNA-OIP5-AS1 was detected by methyl thiazolyl tetrazolium(MTT)colorimetric method.Transwell assay was used to de-tect the migration and invasion ability of shRNA-control and shRNA-OIP5-AS1 cells.The protein expression levels of Vimentin,N-Cadherin,ZEB1 and E-cadherin were detected by enzyme linked immunosorbent assay(ELISA).Independent sample t test was used for inter-group measurement data.Results The mRNA ex-pression of lncRNA OIP5-AS1 in colorectal cancer tissues(1.50±0.13)was significantly higher than that in para-carcinoma tissues(0.54±0.08,t=45.99,P＜0.05).The absorbance of shRNA-OIP5-AS1 cells(0.80±0.06)was significantly lower than that of shRNA-control cells(1.30±0.04,t=16.62,P＜0.05).The number of migrating cells[(74.50±3.73)]and invasion cells[(66.50±5.09)]of shRNA-OIP5-AS1 cells was significantly less than that of shRNA-control cells[(126.33±6.28),(109.67±3.88),t=17.38,16.52,P＜0.05].The expression of E-Cadherin[(5.36±0.28)ng/ml]in shRNA-OIP5-AS1 cells was significantly higher than that in shRNA-control cells[(2.96±0.13)ng/ml,t=18.97,P＜0.05).The protein expression of N-Cadherin[(231.02±27.11)pg/ml],Vimentin[(5.61±0.40)ng/ml]and ZEB1[(2.59±0.28)ng/ml]in shRNA-OIP5-AS1 cells was significantly lower than that in shRNA-control cells[(656.48±39.49)pg/ml,(8.31±0.36)ng/ml,(6.04±0.26)ng/ml,t=21.76,12.32,22.12,P＜0.05].Conclusion The lncRNA OIP5-AS1 expression is up-regulated in colorectal cancer.Knocking down the expression of lncRNA OIP5-AS1 can slow down the proliferation,metastasis and invasion of colorectal cancer cells by inhibiting the epithelial mesenchymal transformation of cells.
Long non-coding RNAColorectal cancerEpithelial-mesenchymal transitionProliferationInvasion
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