Construction of a Bama miniature pig limb ischemia-reperfusion injury model using the method of occluding the iliac artery and vein combined with tourniquet ligation
Objective To establish a simple and effective limb ischemia-reperfusion injury(LI/RI)model in Bama miniature pigs.Methods Totally,12 Bama miniature pigs were randomly divided into a control group(Sham,n=6)and a LI/RI group(n=6),with 6 pigs in each group.The Sham group underwent exposure of blood vessels only,nd the LI/RI group had blood vessels clamped and a tourniquet applied to simulate injury.Oxidative stress,inflammatory response,and tissue injury indicators were com-pared between the two groups using two-way repeated measures ANOVA and t-tests.Results After 7 days,the model was successfully established.The LI/RI group showed significantly higher levels of serum crea-tine kinase(CK),lactate dehydrogenase(LDH),malondialdehyde(MDA),tumor necrosis factor-alpha(TNF-α),creatine kinase-MB(CK-MB),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)than the Sham group(F=8.554,9.167,3.639,10.750,100.336,118.641,5.219,P＜0.05),and lower levels of superoxide dismutase(SOD)(F=2.471,P＜0.05).The LI/RI group had higher levels of muscle MDA,TNF-α,myocardial MDA,and hepatic MDA,TNF-α,IL-6 than the Sham group[(1.93±0.60)nmol/mg prot vs.(0.95±0.24)nmol/mg prot,t=-4.666,P＜0.01;(75.61±44.17)pg/ml vs.(20.65±8.91)pg/ml,t=-2.648,P＜0.05;(2.56±1.03)nmol/mg prot vs.(1.2±0.40)nmol/mg prot,t=-3.192,P＜0.05;(2.88±0.76)nmol/mg prot vs.(0.80±0.28)nmol/mg prot,t=-6.218,P＜0.05;(1 080.79±256.78)pg/ml vs.(441.08±135.50)pg/ml,t=-4.995,P＜0.01;(209.39±94.61)pg/ml vs.(37.18±32.79)pg/ml,t=-3.479,P＜0.05],and lower levels of muscle and myocardial SOD than the Sham group[(87.64±15.13)U/mg prot vs.(114.56±10.41)U/mg prot,t=3.006,P＜0.05;(752.26±118.97)U/mg prot vs.(939.73±90.79)U/mg prot,t=2.777,P＜0.05].Histological examination revealed more severe damage to the skeletal muscle,heart,and liver in the LI/RI group.Conclusion The 6-h vascular clamping combined with a tourniquet can successfully establish the LI/RI model,causing muscle injury,systemic oxidative stress,inflammatory response,and damage to the heart and liver.
Limb ischemia-reperfusion injuryAnimal modelExternal iliac artery and vein ligation
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