Research progress in detection methods of lung organoid model
Lung organoids,formed from adult stem cells or pluripotent stem cells under 3D culture conditions,are gradually emerging as novel tools for studying lung development and pulmonary diseases.Representing a significant breakthrough in biomedical research in recent years,lung organoids have successfully recapitulated the complexity of lung cells and their microenvironment in vitro.As a novel model,lung organoids hold immense potential for investiga-ting lung development and diseases.However,the current detection methods for lung organoids are not yet perfect or standardized,potentially hindering their widespread application.This paper provides a review of commonly used struc-tural and functional detection methods for lung organoids in recent years,aiming to offer guidance for their application.