Objective To prepare novel synthetic hydrogel scaffold materials for peripheral nerve injury repair and evaluate mechanical properties and their in vitro biocompatibility.Methods The novel synthetic hydrogel was prepared by polydimethylsililoxane(polydimethylsililoxane,PDMS),its mechanical properties were detected by universal testing machine,the element compositions of the material was ana-lyzed by X-ray energy spectrometer,and the micromorphology was observed by electron microscope.PC 12 cells were cultured and divided into control group(conventional culture)and experimental group(novel synthetic hydrogel).The cell counting kit-8 assay,cellular immunofluorescence,scratch migration assay and apoptosis assay were used to detect the effects of the novel synthetic hydrogel on the proliferation,mi-gration and apoptosis.The relative expression levels of E-cadherin,Snail,Vimentin,cysteinyl aspartate-specific protease(Caspase)-8,and Caspase-3 were detected by Western blotting to determine the adhesion effects of the novel synthetic hydrogel on cells.The unpaired student's t-test was used to compare two groups.Results The novel synthetic hydrogel had good tensile elasticity,from the initial length 2 cm to 16 cm.Elemental analysis showed that the material was mainly composed of carbon,oxygen and silicon.It had dense uniform mesh structure.The results of cell counting kit-8 assay showed that the absorbance value of PC12 cells in the control group(1.390±0.154)was higher than the novel synthetic hydrogel group(1.093±0.152,t=2.372,P＞0.05).Cellular immunofluorescence results showed that the maturity of PC12 cells in the control group[(68.712±2.877)％]was lower than the novel synthetic hydrogel group[(74.555±2.412)％,t=2.289,P＞0.05].The scratch experiment results showed that the scratch healing area ratio of PC12 cells in the control group[(66.576±3.958)％]was higher than the novel syn-thetic hydrogel group[(64.091±2.635)％,t=1.139,P＞0.05].The apoptosis rate of PC 12 cells in the control group[(10.360±1.807)％]was lower than the novel synthetic hydrogel group[(11.580±1.311)％,t=0.947,P＞0.05].The relative expression levels of E-cadherin,Snail,Vimentin,Caspase-8 and Caspase-3 were lower than the novel synthetic hydrogel group(0.698±0.040 vs.0.785±0.036,0.646±0.050 vs.0.752±0.053,0.759±0.051 vs.0.844±0.051,0.772±0.059 vs.0.836±0.045,0.822±0.024 vs.0.822±0.024,t=2.744,2.539,2.039,1.499,1.525,P＞0.05).Conclusion Novel synthetic hydrogels had good tensile properties and biocompatibility in vitro,and can be used as an option for repairing of peripheral nerve injury.
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