Mechanical properties and in vitro biocompatibility of novel synthetic hydrogel nerve scaffold materials
Objective To prepare novel synthetic hydrogel scaffold materials for peripheral nerve injury repair and evaluate mechanical properties and their in vitro biocompatibility.Methods The novel synthetic hydrogel was prepared by polydimethylsililoxane(polydimethylsililoxane,PDMS),its mechanical properties were detected by universal testing machine,the element compositions of the material was ana-lyzed by X-ray energy spectrometer,and the micromorphology was observed by electron microscope.PC 12 cells were cultured and divided into control group(conventional culture)and experimental group(novel synthetic hydrogel).The cell counting kit-8 assay,cellular immunofluorescence,scratch migration assay and apoptosis assay were used to detect the effects of the novel synthetic hydrogel on the proliferation,mi-gration and apoptosis.The relative expression levels of E-cadherin,Snail,Vimentin,cysteinyl aspartate-specific protease(Caspase)-8,and Caspase-3 were detected by Western blotting to determine the adhesion effects of the novel synthetic hydrogel on cells.The unpaired student's t-test was used to compare two groups.Results The novel synthetic hydrogel had good tensile elasticity,from the initial length 2 cm to 16 cm.Elemental analysis showed that the material was mainly composed of carbon,oxygen and silicon.It had dense uniform mesh structure.The results of cell counting kit-8 assay showed that the absorbance value of PC12 cells in the control group(1.390±0.154)was higher than the novel synthetic hydrogel group(1.093±0.152,t=2.372,P＞0.05).Cellular immunofluorescence results showed that the maturity of PC12 cells in the control group[(68.712±2.877)％]was lower than the novel synthetic hydrogel group[(74.555±2.412)％,t=2.289,P＞0.05].The scratch experiment results showed that the scratch healing area ratio of PC12 cells in the control group[(66.576±3.958)％]was higher than the novel syn-thetic hydrogel group[(64.091±2.635)％,t=1.139,P＞0.05].The apoptosis rate of PC 12 cells in the control group[(10.360±1.807)％]was lower than the novel synthetic hydrogel group[(11.580±1.311)％,t=0.947,P＞0.05].The relative expression levels of E-cadherin,Snail,Vimentin,Caspase-8 and Caspase-3 were lower than the novel synthetic hydrogel group(0.698±0.040 vs.0.785±0.036,0.646±0.050 vs.0.752±0.053,0.759±0.051 vs.0.844±0.051,0.772±0.059 vs.0.836±0.045,0.822±0.024 vs.0.822±0.024,t=2.744,2.539,2.039,1.499,1.525,P＞0.05).Conclusion Novel synthetic hydrogels had good tensile properties and biocompatibility in vitro,and can be used as an option for repairing of peripheral nerve injury.
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