Effect of long non-coding RNA LUCAT1 on proliferation and metastasis of laryngeal carcinoma cells through hypoxia inducible factor-1α
Objective To investigate the effects of long non-coding RNA(lncRNA)LUCAT1 on the proliferation and metastasis of laryngeal carcinoma cells and its molecular mechanism.Methods 39 cases of laryngeal cancer tissues and corresponding adjacent tissues treated in Shangqiu First People's Hospital from June 2022 to December 2023 were selected as research objects,and lncRNA LUCAT1 expression levels in laryngeal cancer tissues and adjacent tissues were analyzed by real-time fluorescence quantitative polymer-ase chain reaction(PCR).Laryngeal cancer cell line AMC-HN-8 was randomly divided into lncRNA con-trol group,lncRNA LUCAT1 KD group and lncRNA LUCAT1 group.LncRNA control group,lncRNA LUCAT1 shRNA and lncRNA LUCAT overexpression plasmid were transfected with liposomes,respective-ly.After transfection for 72 h,the proliferation ability of the three groups of cells was analyzed by cell counting kit-8(CCK-8)and clonal formation assay.The migration and invasion ability of cells were ana-lyzed by scratch test and Traswell test.LncRNA LUCAT binding protein was analyzed by RNA precipitati-on.Protein immune stress analysis of lncRNA LUCAT binding protein level of hypoxia inducible factor-1α(HIF-1α),fluorescent quantitative PCR analysis of HIF-1α target gene expression level.T test was used to compare measurement data between groups.Results The expression level of lncRNA LUCAT1 in larynge-al cancer tissues(1.65±0.23)was significantly higher than that of lncRNA LUCAT1 in paracancer tissues(1.01±0.20),and the difference was statistically significant(t=12.920,P＜0.05).The light absorp-tion value and EDU positive rate of lncRNA control group[1.71±0.12,(74.17±8.13)％]were signifi-cantly higher than those of lncRNA LUCAT1 KD group[0.71±0.09,(45.50±9.35)％],the difference was statistically significant(t=16.490,5.665,P＜0.05).The light absorption value and EDU positive rate of lncRNA control group[1.71±0.12,(74.17±8.13)％]were significantly lower than those of ln-cRNA LUCAT1 group[1.87±0.08,(87.83±2.14)％],the difference was statistically significant(t=2.806,3.980,P＜0.05).The number of migration and invasion cells[(116.83±8.61),(93.17±4.62)cells]in lncRNA control group were significantly higher than those in lncRNA LUCAT1 KD group[(68.33±10.15),(49.33±7.11)cells],the difference was statistically significant(t=8.924,12.650,P＜0.05).The absorption value and EDU positive rate of lncRNA control group[(116.83±8.61),(93.17±4.62)cells]were significantly lower than those of lncRNA LUCAT1 group[(143.83±7.28),(132.83±7.25)cells],the difference was statistically significant(t=5.886,11.300,P＜0.05).HIF-1α protein interacts with lncRNA LUCAT1.The expression levels of VEGF,PDK1 and GLUT1 in lncRNA control group(0.97±0.10,0.93±0.06,1.67±0.11)were significantly higher than those in lncRNA LUCAT1 KD group(0.38±0.10,0.36±0.51,0.51±0.5),the difference was statisti-cally significant(t=10.400,16.170,8.560,P＜0.05).VEGF,PDK1 and GLUT1 expression level[(0.97±0.10),(0.93-0.06),(1.67-0.11)]significantly lower than the lncRNA LUCAT1 group cells(1.55±0.14,1.51±0.11,1.44±0.09,t=8.220,11.140,4.584,P＜0.05).Conclusion Ln-cRNA LUCAT1 is highly expressed in laryngeal cancer tissues.By binding with HIF-1α,lncRNA Lucat1 regulates downstream gene expression,thus affecting the proliferation and metastasis of laryngeal cancer cells.
Long non-coding RNAHypoxia-inducible factor-1 αlaryngeal carcinomaProliferationTumor metastasis
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