Objective To explore the protective effect and molecular mechanism of Panax notogin-seng saponins on acute kidney injury in rats.Methods Totally,30 SD rats were randomly divided into a control group,a model group,and a notoginseng saponin group,with 10 rats in each group.The rats in the model group and notoginseng saponin group were given a one-time gavage of 2 g/kg acetaminophen to estab-lish acute kidney injury models.At 24 h after successful modeling,the rats in the notoginseng saponin group were intraperitoneally injected with 100 mg/kg notoginseng saponin,and those in the control group and model group were intraperitoneally injected with equal volume of physiological saline.The renal index of three groups after 10 days of treatment was analyzed.The pathological changes in the kidneys of three groups were analyzed by hematoxylin eosin(HE)staining.The serum creatinine and urine nitrogen levels were determined using a reagent kit.The levels of serum oxidative stress indicators such as malondialde-hyde(MDA),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)in three groups were measured by Biochemical methods.The levels of serum inflammatory factors[interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)]in three groups were detected by enzyme linked immunosorbent assay(ELISA).The expression levels of phosphorylated p38 mitogen-activated protein kinase(p-p38 MAPK),phosphorylated extracellular signal-regulated kinase(p-ERK),and phosphorylated c-Jun N-terminal kinase(p-JNK)proteins in the renal tissues of three groups were detected by Western blotting.The comparison of inter group econometric data was conducted using one-way analysis of variance.Results The renal index of the rats in the Panax notoginseng saponin group(0.95±0.04)was significantly lower than that in the model group(1.13±0.07,t=7.547,P＜0.05).Serum creatinine and urine nitrogen in rats treated with Panax notoginseng saponins[(47.31±4.40)and(18.65±1.88)mmol/L]were significantly lower than those in the model group[(76.52±5.34)and(26.31±2.74)mmol/L,t=13.350,7.288,P＜0.05].The renal pathological score in the Panax notoginseng saponin group(1.60±0.70)was significantly lower than that in the model group(3.80±0.63,t=7.379,P＜0.05).SOD activity and GSH-Px levels in kid-ney tissue of rats treated with Panax notoginseng saponins[(217.37±13.29)and(1.60±0.09)μmol/L]were significantly higher than in the model group[(142.33±18.88)and(1.18±0.11)μmol/L,t=10.280,9.457,P＜0.05].The renal MDA levels in the Panax notoginseng saponin group[(0.80±0.07)nmol/mg]were significantly lower than those in the model group[(1.44±0.14)nmol/mg,t=12.830,P＜0.05].The serum levels of TNF-α and IL-6 in the Panax notoginseng saponin group[(67.96±9.84)and(91.92±6.63)pg/ml]were significantly lower than those in the model group[(67.96±9.84)and(91.92±6.63)pg/ml,t=9.384,14.020,P＜0.05].The expression levels of p-p38 MAPK,p-ERK,and p-JNK proteins in the kidney tissue of rats in the Panax notoginseng saponin group[(1.21±0.08,1.16±0.14,1.32±0.13)were significantly lower than those in the model group(1.74±0.14,1.55±0.16,1.94±0.09,t=10.280,5.887,12.460,P＜0.05).Conclusion Panax notoginseng saponins can reduce the release of inflammatory factors,improve renal injury,and protect renal function by inhibiting the expression of MAPK signaling pathway.
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