The role of genipin in ischemia-reperfusion injury in rat kidney transplantation
Objective To investigate the action mechanism of genipin on ischemia-reperfusion in-jury in rat kidney transplantation.Methods Totally,36 healthy adult male SD rats were divided into four groups according to the randomized numerical table method:the control group(Control),the sham opera-tion group(Sham),the kidney transplantation group(KT),and the genipin intervention group(GP),with 6 rats in each of the Control and Sham groups,and 6 rats in each of the donor and recipient groups of the KT and GP groups.The rat in situ kidney transplantation model was used,and saline gavage was given to the Sham and KT groups 1 h before operation,and 50 mg/kg of genipin was given to the GP group 1 h be-fore operation.After 24 h of modeling,hematoxylin-eosin(HE)staining was used to observe the pathologi-cal results of renal tissue.The enzyme-linked immunosorbent assay(ELISA)was used to detect serum cre-atinine(Scr)and blood urea nitrogen(BUN)levels,superoxide dismutase(SOD)and malondialdehyde(MDA)levels in the rat kidney tissue.The notched-end-labeling method of terminal deoxynucleotidyl transferase dUTP transferase(TUNEL)was used to detect apoptosis.The protein expression of adenylate-activated protein kinase(AMPK),silencing information regulator-1(SIRT1),and uncoupling protein 2(UCP2)was detected in renal tissues by protein immunoblotting(Western blotting).The t-test was used for comparison between groups.Comparison of continuous data between two groups was performed by inde-pendent samples t-test.Results HE results showed that the kidneys of rats in the KT group had inflamma-tory cell infiltration,tubular atrophy and degeneration,interstitial cell edema,and cortical hemorrhage,while the GP group had less structural damage to the renal tissues.ELISA results showed that serum Scr,BUN,and MDA of renal tissues in the GP group were lower than those of the KT group(42.06±4.03 vs.53.18±3.59,28.82±2.31 vs.42.49±5.05,4.01±0.39 vs.5.13±0.31,t=5.04,6.03,5.42,P＜0.05),while SOD level was higher than that in the KT group(8.58±0.75 vs.6.88±0.45,t=4.77,P＜0.05).The results of the TUNEL assay showed that the apoptosis rate of renal tissues of rats in the GP group was lower than that in the KT group(3.53±1.795 vs.17.53±6.91,t=4.81,P＜0.05).Western blotting results showed that AMPK and SIRT1 protein expression levels were higher in the GP group than in the KT group(1.07±0.17 vs.0.52±0.11,0.84±0.15 vs.0.48±0.23,t=6.49,3.29,P＜0.05),whereas the UCP2 protein expression level was significantly lower than that in the KT group(0.85±0.20 vs.1.18±0.07,t=3.89,P＜0.05).Conclusion Genipin may attenuate ischemi-a-reperfusion injury in transplanted kidneys by activating the AMPK/SIRT1 pathway.
GenipinKidney transplantationReperfusion InjuryAMP-activated protein kinase/Silent information regulator1/Uncoupling protein 2Inflammatory factors
NETL
NSTL
万方数据
