Effect of bone marrow stromal cell antigen 2 on metastatic activity of papillary thyroid cancer cells
Objective To explore the effect of marrow stromal antigen protein 2(BST2)on prolif-eration,metastasis and apoptosis papillary thyroid cancer cells by regulating phosphatidyl inositol 3 kinase/serine and threonine kinase(PI3K/Akt)signal pathway.Methods Cancer tissues and adjacent tissues were collected from 11 patients(65±8 years old)with papillary thyroid carcinoma diagnosed and treated in Shanxi Cancer Hospital from August 2022 to November 2023.Thyroid papillary carcinoma TPC-1 cells were randomly divided into 4 groups by the random number table method:negative control group,si BST2 group,PI3K/Akt signaling pathway inhibitor group,pcDNA BST2 group.Immunohistochemistry was used to analyze the expression level of BST2 in papillary thyroid carcinoma tissues and adjacent tissues.The pro-liferation ability of TPC-1 cells in each group was analyzed by CCK-8 assay.The migration ability of TPC-1 cells in each group was analyzed by cell scratch test.Transwell assay was used to analyze the invasion ability of TPC-1 cells in each group.The apoptosis rate of TPC-1 cells in each group was analyzed by flow cytome-try.The expression of BST2 and PI3K/Akt pathway proteins in TPC-1 cells were analyzed by Western blotting.Independent sample t test was used for comparison between the two groups.Results The expres-sion of BST2 in papillary thyroid carcinoma tissues was higher than that in adjacent tissues(0.17±0.03 vs.0.83±0.05,t=12.905,P＜0.05).The expression of BST,Akt,PI3K proteins in TPC-1 cells of siBST2 group was lower than in the negative control group(0.60±0.02 vs.0.13±0.02,t=11.367,P＜0.05;0.59±0.05 vs.0.11±0.03,t=9.235,P＜0.05;0.51±0.06 vs.0.15±0.03,t=15.325,P＜0.05).The protein expression of BST,Akt and PI3K in TPC-1 cells of pcDNA BST2 group was higher than that of negative control group(0.60±0.02 vs.0.88±0.55,t=11.911,P＜0.05;0.59±0.05 vs.0.81±0.03,t=16.255,P＜0.05;0.51±0.06 vs.0.82±0.03,t=13.271,P＜0.05).Proliferation,migration and invasion ability of TPC-1 cells in siBST2 group and PI3K/Akt signal pathway inhibitors group was lower lower than in the negative control group(85.12±8.12 vs.153.28±9.88,t=12.766,P＜0.05;81.62±9.33 vs.153.28±9.88,t=11.698,P＜0.05),the apoptosis rate of TPC-1 cells in the siBST2 group and the PI3K/Akt signaling pathway inhibitor group was higher than that in the negative con-trol group(13.05±1.16 vs.6.70±0.12,t=9.081,P＜0.05;14.36±0.98 vs.6.70±0.12,t=11.661,P＜0.05).The proliferation,migration and invasion abilities of TPC-1 cells in the pcDNA BST2 group were higher than those in the negative control group(209.78±15.92 vs.153.28±9.88,t=12.568,P＜0.05).The apoptosis rate of TPC-1 cells in pcDNA BST2 groups was lower than in the nega-tive control group(3.56±0.05 vs.6.70±0.12,t=12.562,P＜0.05).Conclusion Down-regulating BST2 or inhibiting PI3K/Akt signaling pathway can inhibit proliferation,migration and invasion ability of TPC-1 cells and promote apoptosis of TPC-1 cells.
Bone marrow stromal cell antigen 2Papillary thyroid carcinomaPhosphatidyl inositol 3 kinaseSerine and threonine kinaseProliferationMetastasisApoptosis
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