Proteomic analysis of potential S-palmitoylation in colorectal cancer
Objective To identify S-palmitoyl protein in colon cancer by mass spectrometry,and to provide theoretical basis for finding a new target for clinical treatment of colorectal cancer.Methods Colorectal cancer tissues of 11 patients(7 males and 4 females)obtained from the Second Hospital of Jilin University from December 2022 to March 2023 were selected.At the same time,acyl-biotinyl exchange(ABE)was performed with colorectal cancer cell lines for identification by mass spectrometry,and the po-tential S-palmitoylation proteins were analyzed and verified by Western blotting.Paired sample t test was used for comparison between the two groups.Results There were 21 proteins in human colorectal cancer tissues and SW480 and SW620 cell lines that shared potential S-palmitoylation sites,and these proteins were closely related to immune regulation by gene ontology(GO)and Pathway analysis.Western blotting confirmed that phospholipid scramblase 1(PLSCR1)and cytoskeleton-associated protein 4(CKAP4)were S-palmitoylation.The total CKAP4 protein level in human colorectal cancer tissues(0.693±0.092,1.162±0.003,0.771±0.012,1.683±0.085,1.144±0.266)was significantly lower than that in adja-cent tissues(2.306±0.231,1.471±0.055,1.201)±0.01,1.978±0.031,2.107±0.035)(t=11.260,9.744,15.260,5.398,6.221,P＜0.05).The total protein level of PLSCR1(3.185±0.047,1.305±0.153,2.131±0.0752,2.542±0.106)was significantly higher than that in para-cancerous tis-sues(0.833±0.0529,1.038±0.057,1.477±0.095,2.108±0.034)(t=21.372,28.770,13.559,11.460,P＜0.05).Conclusion Colorectal cancer contains potential S-palmitoylation modified proteins that may influence the proliferation of colorectal cancer by modulating immune function.
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