Correlation between signal transducer and activators of transcription 3 and lymph node microme-tastasis in early-stage non-small cell lung cancer and its clinical significance
Objective To explore the relationship between signal transducer and activators of tran-scription 3(STAT3)expression and lymph node micrometastasis in early stage non-small cell lung cancer(NSCLC)and its effect on the prognosis of patients,so as to discover new mechanisms of tumor microme-tastasis and targeted drugs against STAT3 pathway.Methods Lung tissue and lymph node samples were collected from 50 patients with early-stage NSCLC and 50 patients with benign lung tumors who underwent partial lung resection at Shandong Provincial Hospital Affiliated to Shandong First Medical University from June 2008 to January 2010.Reverse transcription polymerase chain reaction was used to detect the expres-sion of MUC1 mRNA in lymph node samples and STAT3 mRNA in NSCLC samples and normal lung tissue samples.The relationship between the expression of MUC1 mRNA and STAT3 mRNA and the clinicopatho-logical characteristics of NSCLC patients was analyzed.Western blotting and immunohistochemistry was used to detect the expression of STAT3 and pSTAT3 in NSCLC samples and normal lung tissue samples,and the relationship between STAT3 and pSTAT3 protein expression and clinicopathological characteristics of patients was analyzed.The Kaplan-Meier method was applied to plot survival curves,and Logistic re-gression analysis was used to determine the independent risk factors for lymph node micrometastasis.Results The expression of STAT3 mRNA and protein was correlated with lymph node micrometastasis.The expression of STAT3 mRNA in MUC 1 positive group was higher than that in negative group(0.950±0.100 vs.0.711±0.080,t=9.308,P＜0.05).Additionally,STA13 protein expression in MUC 1 posi-tive group was higher than that in negative group(1.086±0.078 vs.0.860±0.085,t=9.427,P＜0.05).There was no significant difference in STAT3 expression in the prognosis of NSCLC patients(x2=2.350,P＞0.05).Logistic regression analysis showed that STAT3 protein overexpression[odds ratio(OR)=13.379,P＜0.05]and tumor differentiation(OR=0.131,P＜0.05)were independent risk fac-tors for lymph node micrometastasis.Conclusion STAT3 overexpression may promote lymph node micro-metastasis in early stage NSCLC,and may be used as a clinical predictor of lymph node micrometastasis.
Non-small cell lung cancerIymph node metastasisSignal transducer and activa-tors of transcription 3phosphorylated signal transducer and activators of transcription 3MUC1
NETL
NSTL
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