Relationship between immune function and hip function in patients with hip joint bone defect after operation
Objective To investigate the relationship between immune function,cytokine level and hip joint function in patients with hip joint defect after surgery.Methods A total of 33 patients with hip bone defect admitted to The Second People's Hospital of Liaocheng City from December 2021 to December 2023 were selected as the study objects,all of whom underwent total hip revision through posterolateral ap-proach.Venous blood was drawn before and in the morning after surgery to separate serum and blood cells.T lymphocyte subsets(CD3+,CD4+,CD8+,CD4+/CD8+,TH1/TH2,Treg)and natural killer(NK)cells were analyzed by flow cytometry and Harris hip function scale was used to analyze the hip function of patients with hip defect before and after surgery.Correlation analysis was used to analyze the relationship between the proportion of immune cells(CD3+,CD4+,CD8+and CD4+/CD8+)and inflammatory fac-tors[interleukin(IL)-4,IL-10 and interferon-γ(IFN-γ)]and Harris score.T test was used to compare measurement data between groups.Results After surgery,the proportion of CD3+,CD4+,and CD8+lymphocytes and the CD4+/CD8 ratio in patients with hip bone defects were significantly lower than the preoperative values[(80.93±6.56)％,(53.20±5.29)％,(26.93±2.93)％,1.58±0.05 vs.(72.80±5.32)％,(45.53±6.56)％,(22.73±2.12)％,1.25±0.10,t=7.903,7.340,7.171,17.610,P＜0.05].The Th1/Th2 ratio and the proportion of NK cells in patients with hip bone defects were significantly lower than the preoperative values[1.23±0.08,(13.12±1.92)％vs.1.49±0.17,(17.16±1.74)％,t=7.928,8.763,P＜0.05].The proportion of regulatory T cells in patients with hip bone defects was significantly higher than the preoperative value[(7.06±0.64)％vs.(3.53±0.33)％,t=27.900,P＜0.05].The levels of IL-4 and IL-10 in patients with hip bone defects after surgery[(52.03±10.13),(43.42±8.56)pg/ml]were significantly higher than the preoperative levels[(35.57±6.79),(30.27±4.89)pg/ml,t=7.746,7.665,P＜0.05].The level of INF-y inpatients with hip bone defects after surgery[(43.00±8.78)pg/ml]was significantly higher than the preoperative level[(22.30±3.75)pg/ml,t=7.746,P＜0.05].The preoperative hip joint function score of the pa-tients[(72.15±7.94)points]was significantly lower than the postoperative hip joint score of the patients[(91.75±4.48)points,t=12.340,P＜0.05].Harris hip function score was negatively correlated with CD3+,CD4+,CD8+lymphocyte proportion and CD4+/CD8 ratio(r=-0.891,-0.778,-0.715,P＜0.05).Treg proportion,IL-4 and IL-10 levels were negatively correlated with Harris hip function score(r=-0.521,-0.891,-0.778,P＜0.05).Th1/TH2 ratio,NK cell proportion,and INF-y level were nega-tively correlated with Harris hip function score(r=0.441,0.285,0.514,P＜0.05).Conclusion The im-mune function of patients with hip joint defect after hip joint replacement is significantly improved,and the immune function of patients after hip joint replacement is positively correlated with the hip joint function.
Hip bone defectHip replacementInflammatory factorsImmune function
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