Objective To explore the metabolic characteristics of Dupuytren's Disease(DD)at the level of metabolomics using liquid chromatograph-mass spectrometer(LC/MS)technology,and to screen for differential metabolites,providing a theoretical basis for target verification.Methods The con-tracted palmar aponeurosis tissue resected during DD surgery in Baogang Hospital of Inner Mongolia from January 2019 to December 2022 was collected as the experimental group,and 20 cases of normal palmar aponeurosis tissue resected during carpal tunnel syndrome surgery in the same period were selected as the control group.Using LC/MS technology,the metabolites in the two groups were analyzed qualitatively and quantitatively,and the metabolic differences between the two groups were revealed by principal component analysis and partial least squares discriminant analysis,hierarchical clustering was performed,and valuable differential metabolites were screened out by the P value,the VIP coefficient,and the effective threshold setting of FC value.Box-and-line diagrams,volcano diagrams,chord diagrams,bubble diagrams,and KEGG pathway diagrams were drawn to observe the intergroup differences and metabolic pathway enrich-ment,and the correlation analysis of each metabolite was completed by Pearson correlation coefficient.T test was used for comparison between the two groups.Results(1)A total of 931 metabolites were iden-tified in 40 samples using LC/MS technology,of which 439 had significantly different expression levels(P＜0.05,VIP＞1.00,FC＜0.50 or FC＞2.00).(2)After KEGG pathway annotation,differential me-tabolites were enriched in 42 pathways(x/n＞y/N,x:number of pathway-associated differential metabo-lites;y:number of pathway-associated total metabolites;n:number of annotated differential metabolites;N:number of annotated total metabolites).Conclusion Abnormal manifestations of DD in the three path-ways of carbon metabolism,unsaturated fatty acid biosynthesis,and cysteine and methionine metabolism demonstrate that the pathogenic mechanism is related to inflammation,oxygen free radicals,diabetes melli-tus,and local ischemia and hypoxia.
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