Objective To investigate the effect of small molecule agonist Yoda1 on the expression of transcriptome genes in(mouse embryo osteoblast precursor cells)MC3T3-E1 and analyze possible func-tions and pathways.Methods The experiment was divided into control group which untreated and Yoda1 group with Yoda1 treated.Transcriptome sequencing was performed after treatment of MC3T3-E1 cells with Yoda1 for 24 hours.The differentially expressed genes obtained were used for Gene Set Enrichment Analy-sis(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Set Enrichment Analysis(GSEA).R was used for statistical analysis.Results 936 differentially expressed genes were obtained,of which 568 were down-regulated and 368 were up-regulated.GO analysis revealed that DEGs in biological process(BP)was mainly involved in cellular processes(708),biological regulation(581),biological process regulation(574),metabolic processes(450),response to stimuli(421).DEGs in cellular compo-nent(CC)was mainly involved in protein complexes(294).DEGs in molecular function(MF)was mainly involved in binding(610)and catalytic activity(273).DEGs was mainly associated with organelle fission(11.52％,93/807),nuclear division(11.15％,90/807),chromosome segregation(10.16％,82/807),and nuclear chromosome segregation(8.43％,68/807)(P＜0.05).KEGG analysis showed that cell cycle(8.06％,29/360),DNA replication(5.28％,19/360),Fanconi anemia pathway(3.89％,14/360),homologous recombination(3.61％,13/360),and mismatch repair(1.94％,7/360)were the main path-ways that Yodal affected MC3T3-E1 cells(P＜0.05).GSEA analysis showed a significant upregulation of the E-BOX binding in Yoda1 group(P＜0.05,FDR q-val＜25％,NES=-1.923).Conclusion Yoda1 has an impact on the growth of MC3T3-E1 cells and upregulates E-BOX binding.
Piezo type mechanosensitive ion channel component 1BioinformaticsE-BOX bindingMouse embryo osteoblast precursor cells
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