首页|小分子激动剂Yoda1对小鼠胚胎成骨前体细胞转录组表达的影响

小分子激动剂Yoda1对小鼠胚胎成骨前体细胞转录组表达的影响

扫码查看
目的 探讨小分子激动剂Yoda1对小鼠胚胎成骨前体细胞(MC3T3-E1)转录组基因表达的影响,分析可能的功能和通路.方法 实验分为未经处理的对照组和Yoda1干预的Yoda1组,Yoda1处理MC3T3-E1细胞24 h后,进行转录组测序,筛选差异表达基因进行基因本体论(GO)富集分析、京都基因与基因组数据(KEGG)富集分析,并使用基因集富集分析(GSEA),明确差异基因主要富集的功能和通路等,使用R软件进行生物信息学分析.结果 经筛选,共有936个差异表达基因,其中568个下调,368个上调.GO分析显示,DEGs在BP中主要涉及细胞过程(708个)、生物调控(581个)、生物过程调控(574个)、代谢过程(450个)、对刺激的响应(421个)等,DEGs在CC中主要涉及蛋白质复合体(294个),DEGs在MF中主要涉及结合(610个)、催化活性(273个)等.DEGs主要和细胞器分裂(11.52%,93/807)、核分裂(11.15%,90/807)、染色体分离(10.16%,82/807)和核染色体分裂(8.43%,68/807)等有关(P<0.05).KEGG分析显示细胞周期(8.06%,29/360)、DNA 复制(5.28%,19/360)、范可尼贫血通路(3.89%,14/360)、同源重组(3.61%,13/360)和错配修复(1.94%,7/360)等是Yoda1影响MC3T3-E1细胞的主要通路(P<0.05).GSEA分析显示 Yoda1 组 E-BOX 结合显著上调(P<0.05,FDR q-val<25%,NES=-1.923).结论 Yoda1与MC3T3-E1细胞生长有关,且上调E盒结合.
The effects of small molecule agonist Yoda1 on the transcriptome gene expression of mouse embryo osteoblast precursor cells
Objective To investigate the effect of small molecule agonist Yoda1 on the expression of transcriptome genes in(mouse embryo osteoblast precursor cells)MC3T3-E1 and analyze possible func-tions and pathways.Methods The experiment was divided into control group which untreated and Yoda1 group with Yoda1 treated.Transcriptome sequencing was performed after treatment of MC3T3-E1 cells with Yoda1 for 24 hours.The differentially expressed genes obtained were used for Gene Set Enrichment Analy-sis(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Set Enrichment Analysis(GSEA).R was used for statistical analysis.Results 936 differentially expressed genes were obtained,of which 568 were down-regulated and 368 were up-regulated.GO analysis revealed that DEGs in biological process(BP)was mainly involved in cellular processes(708),biological regulation(581),biological process regulation(574),metabolic processes(450),response to stimuli(421).DEGs in cellular compo-nent(CC)was mainly involved in protein complexes(294).DEGs in molecular function(MF)was mainly involved in binding(610)and catalytic activity(273).DEGs was mainly associated with organelle fission(11.52%,93/807),nuclear division(11.15%,90/807),chromosome segregation(10.16%,82/807),and nuclear chromosome segregation(8.43%,68/807)(P<0.05).KEGG analysis showed that cell cycle(8.06%,29/360),DNA replication(5.28%,19/360),Fanconi anemia pathway(3.89%,14/360),homologous recombination(3.61%,13/360),and mismatch repair(1.94%,7/360)were the main path-ways that Yodal affected MC3T3-E1 cells(P<0.05).GSEA analysis showed a significant upregulation of the E-BOX binding in Yoda1 group(P<0.05,FDR q-val<25%,NES=-1.923).Conclusion Yoda1 has an impact on the growth of MC3T3-E1 cells and upregulates E-BOX binding.

Piezo type mechanosensitive ion channel component 1BioinformaticsE-BOX bindingMouse embryo osteoblast precursor cells

张心毅、刘暾、赵家鑫、王伟、宋继东

展开 >

西安交通大学第二附属医院骨科,西安 710003

压电型机械敏感离子通道组件1 生物信息学 E盒结合 小鼠胚胎成骨前体细胞

2024

中华实验外科杂志
中华医学会

中华实验外科杂志

CSTPCD
影响因子:0.759
ISSN:1001-9030
年,卷(期):2024.41(11)