The effects of small molecule agonist Yoda1 on the transcriptome gene expression of mouse embryo osteoblast precursor cells
Objective To investigate the effect of small molecule agonist Yoda1 on the expression of transcriptome genes in(mouse embryo osteoblast precursor cells)MC3T3-E1 and analyze possible func-tions and pathways.Methods The experiment was divided into control group which untreated and Yoda1 group with Yoda1 treated.Transcriptome sequencing was performed after treatment of MC3T3-E1 cells with Yoda1 for 24 hours.The differentially expressed genes obtained were used for Gene Set Enrichment Analy-sis(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Set Enrichment Analysis(GSEA).R was used for statistical analysis.Results 936 differentially expressed genes were obtained,of which 568 were down-regulated and 368 were up-regulated.GO analysis revealed that DEGs in biological process(BP)was mainly involved in cellular processes(708),biological regulation(581),biological process regulation(574),metabolic processes(450),response to stimuli(421).DEGs in cellular compo-nent(CC)was mainly involved in protein complexes(294).DEGs in molecular function(MF)was mainly involved in binding(610)and catalytic activity(273).DEGs was mainly associated with organelle fission(11.52%,93/807),nuclear division(11.15%,90/807),chromosome segregation(10.16%,82/807),and nuclear chromosome segregation(8.43%,68/807)(P<0.05).KEGG analysis showed that cell cycle(8.06%,29/360),DNA replication(5.28%,19/360),Fanconi anemia pathway(3.89%,14/360),homologous recombination(3.61%,13/360),and mismatch repair(1.94%,7/360)were the main path-ways that Yodal affected MC3T3-E1 cells(P<0.05).GSEA analysis showed a significant upregulation of the E-BOX binding in Yoda1 group(P<0.05,FDR q-val<25%,NES=-1.923).Conclusion Yoda1 has an impact on the growth of MC3T3-E1 cells and upregulates E-BOX binding.
Piezo type mechanosensitive ion channel component 1BioinformaticsE-BOX bindingMouse embryo osteoblast precursor cells