The effect of harmine derivative BCN-3-5 on the malignant features of triple-negative breast cancer
Objective To investigate the effects of BCN-3-5 on cell proliferation,apoptosis,migration,invasion in triple negative breast cancer(TNBC)cells.Methods Sixty MDA-MB-231 and MDA-MB-468 triple-negative breast cancer cells were procured as research objects.Both the MDA-MB-231 and MDA-MB-468 cell lines were randomly divided into 5 groups each through the simple random grouping method,12 plants per group.The cells were intervened with drugs and classified into a blank control group,a BCN-3-5 group,a cisplatin group,an SAJM589 group,and a 10058-F4 group.Cell proliferation was detected using the cell viability assay kit(CCK-8 method),cell migration and invasion were detected using the Transwell assay,and cell apoptosis was detected by flow cytometry.100 zebrafish tumor trans-plantation model was established and randomly divided into 3 groups with 20 individuals in each group,namely a model blank control group,a model cisplatin group,and a model BCN-3-5 group.The anti-tumor growth and apoptosis effects of BCN-3-5 on breast cancer were analyzed by measuring the fluorescence in-tensity of tumor cells.The statistical results were expressed as mean±standard deviation((x)±s),and the comparison of group means was conducted using independent sample t-tests and non-parametric tests.Results The CCK8 results showed that the survival rate of BCN-3-5 group was significantly higher than that of the blank control group[(77.001±6.261)％vs.(99.999±7.652)％,t=5.213,P＜0.05],with a statistically significant difference.The results of the Transwell assay showed that the number of mi-grating cells in the BCN-3-5 group was lower than that in the blank control group(142.000±18.193 vs.255.000±24.000,t=6.499,P＜0.05);the number of invasive cells in the BCN-3-5 group was lower than that in the blank control group(124.333±22.368 vs.249.667±17.039,t=7.720,P＜0.05),with a statistically significant difference.The flow cytometry results showed that the rate of cell apoptosis in the BCN-3-5 group was higher than that in the blank control group[(10.913±1.432)％vs.(3.313±0.351)％,t=8.928,P＜0.05],with a statistically significant difference.The zebrafish xenograft tumor model experiment results showed that the inhibitory effect of the model BCN-3-5 group on tumor growth and the promotion of tumor apoptosis was stronger than that of the model blank control group[(246 826±276 370)pixels vs.(419 489±47 545)pixels,t=9.929,P＜0.05;(1 689 918±58 288)pixels vs.(1 307 668±38 241)pixels,t=17.34,P＜0.05];the inhibitory effect of the model BCN-3-5 group on tumor growth and the promotion of tumor apoptosis was stronger than that of the model cisplatin group[(246 826±27 637)pixels vs.(285 347±40 576)pixels,t=2.481,P＜0.05;(1 689 918±58 288)pixels vs.(1 601 809±93 033)pixels,t=2.538,P＜0.05],with a statistically significant difference.Conclusion Harmine derivativeBCN-3-5 can inhibit the malignant characteristics of triple neg-ative breast cancer.
Triple-negative breast cancerHarmine derivativesBCN-3-5c-Myc
NETL
NSTL
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