Analysis of the inhibitory effect of soapberry thorn on the proliferation of human colon cancer HCT116 cells based on network pharmacology
Objective Based on network pharmacology,molecular docking,and in vitro experi-ments,this study aims to reveal the potential mechanisms of Gleditsiae Spina in the treatment of colorectal cancer.Methods The drug targets of soapberry thorn are derived from the Traditional Chinese Medicine Systems Pharmacology(TCMSP)platform.The targets of colon cancer-related diseases are sourced from The Human Gene Database(Genecards)and the Online Mendelian Inheritance in Man(OMIM)database.Obtain the intersection targets of the two through Venn diagram,and further explore potential mechanisms through Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes KEGG,and network analysis.Verify the reliability of the effect of soapberry thorn on colon cancer-related target proteins through in vitro proliferation experiments(RT-qPCR).Use one-way ANOVA for inter group comparison.The measurement data is expressed as mean±standard deviation((x)±s),and independent-samples t-test is used for com-parison between two groups,while analysis of variance is used for comparison between multiple groups.Re-sults Retrieve soapberry thorns from the TCMSP database and screen out 113 non duplicated targets corre-sponding to the active ingredients;201 colon cancer-related targets were screened from Genecards and OMIM databases;The above-mentioned soapberry thorn drug targets and colon cancer-related disease tar-gets were plotted on a Venn diagram using a WeChat online drawing tool,and 23 common drug disease tar-gets were screened;The 23 therapeutic targets obtained were selected through PPI network construction and enrichment analysis to identify the core targets of colon cancer as follows:matrix metalloproteinase(MMP)-1,MMP-9,reticuloendotheliosis virus oncogene homolog A recombinant protein(RELA),and prostaglan-din endoperoxide synthase 2(PTGS2).The results of in vitro cell experiments showed that the proliferation level of HTC116 cells in the soapberry thorn treated group was significantly higher than that in the control group and formed a dose-dependent relationship(180.72±16.51 vs.110.20±14.68,t=0.096,P＜0.05);After treating HTC116 cells with 50 μmol/L soapberry thorns for 24 hours,the expression levels of MMP-1,MMP-9,RELA,and PTGS2 in the drug treatment group were significantly lower than those in the control group(120.62±72.33,116.62±73.21,130.57±69.46,124.15±58.35 vs.152.48±69.98,160.31±67.54,171.25±63.62,177.03±70.72,t=0.082,0.076,0.081,0.079,P＜0.05).Con-clusion Zaojiao thorn can inhibit the proliferation of colon cancer HCT116 cells,and its mechanism of ac-tion may be related to improving inflammatory response.
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