Protective effect of tanshinone Ⅱ on rats with acute renal failure
Objective To investigate the protective effect of tanshinone Ⅱ on acute renal failure rats and its molecular mechanism.Methods 45 SD rats were randomly divided into control group,renal failure group and tanshinone group by random number table method(10 mice/group).The rats in renal failure group and tanshinone group were injected with 10 mg/kg lipopolysaccharide intritoneally to establish acute renal failure rat model,and the rats in control group were injected with equal volume normal saline.After successful modeling,the rats in the tanshinone group were intraperitoneally injected with 30 mg/kg tanshinone Ⅱ,and the rats in the control group and the renal failure group were injected with equal volume normal saline.After 7 days of treatment,hematoxylin-eosin staining was used to analyze renal pathological changes in the three groups.Serum creatinine(Scr),uric acid(UA),cystatin C(Cys C)and blood and urine nitrogen(BUN)levels were detected in the three groups.The apoptosis level of renal tissue was ana-lyzed by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)staining.Serum in-flammatory factors were analyzed by enzyme-linked immunosorbent assay(ELISA).The expression levels of protein kinase B(Akt)/phosphatidylinositol 3 kinase(PI3K),cysteinyl aspartate-specific protease-3(Caspase-3)and B cell lymphoma/leukemia-2 associated X protein(bax)were analyzed by Western blot-ting.One-way analysis of variance was used to compare the measurement data between groups.Results The pathological score of kidney tissue in tanshinone group[(1.27±0.70)points]was significantly lower than that in renal failure group[(3.13±0.74)points],and the difference was statistically significant(t=15.100,P＜0.05).Scr,BUN,UA and Cys C in tanshinone group[(0.76±0.09)μmol/L,(72.20±12.73)mmol/L,(232.93±18.66)μmol/L,(0.33±0.05)mg/L]was lower than that in group renal failure rats[(1.22±0.08)μmol/L,(118.00±19.87)mmol/L,(375.53±28.20)μmol/L,(0.49±0.06)mg/L,t=15.260,7.516,8.048,16.330,P＜0.05].Serum interleukin-6 and tumor necrosis factor[(70.07±7.76),(55.67±10.73)ng/ml]in tanshinone group were significantly lower than those in renal failure group[(130.60±20.79),(85.33±8.36)ng/ml,t=10.560,8.443,P＜0.05].The apoptosis index of renal tissue in tanshinone group[(14.02±1.98)％]was significantly lower than that in renal failure group[(33.30±6.67)％,t=10.730,P＜0.05].The protein expression levels of phospho-rylated Akt and phosphorylated PI3K in the renal tissue of rats in the tanshinone group(0.83±0.07,0.80±0.06)were significantly higher than those in the renal failure group(0.57±0.09,0.44±0.11,t=11.750 12.630,P＜0.05).The protein expression levels of Caspase-3 and bax in the renal tissue rats in the tanshinone group(0.70±0.07,0.76±0.08)were significantly than those in the renal failure group(0.99±0.10,1.19±0.08,t=9.468,14.030,P＜0.05).Conclusion Danshensu Ⅱ has a protective effect on acute renal failure induced by lipopolysaccharide,reducing inflammatory response and cell apopto-sis,mainly through the Akt/PI3K signaling pathway.
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