Neuroprotective effects and molecular mechanisms of Scutellarin on rats with Glaucoma
Objective To explore the neuroprotective effects and molecular mechanisms of Scutel-larin on rats with glaucoma.Methods 30 SD rats were divided into control group,glaucoma group,and Scutellarin group by ransom number table methods(10 mice/group).The glaucoma group and Scutellarin group were established as glaucoma models using 532-diode laser photocoagulation,while the control group served as the.After the model was successfully established,the Scutellarin group was intraperitoneally in-jected with Scutellarin 0 mg/kg once a day;the control group and the glaucoma group were intraperitoneally injected with an equal volume of physiological saline.After weeks of treatment,the changes in intraocular pressure of the three groups of rats were measured.The changes and thickness changes in the retinas of the three groups of rats were analyzed by hematoxylin and eosin staining.The number of retinallion cells in the three groups of rats were analyzed by immunofluorescence.The apoptosis index in the retinal tissues of the rats were analyzed by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)staining.The expression levels of inflammatory factors in the retinal tissues of the three groups of rats were analyzed by fluorescence quantitative polymerase chain reaction(PCR).The expression levels of phosphorylated c-Jun N-terminal kinase(p-JNK),p-p38 mitogen-activated protein kinase(MAPK),cysteinyl aspartate-specific protease-3(Caspase-3),and B cell lymphoma/leukemia-2 associated X protein(bax)proteins in the retinal tissues of the rats were analyzed by Western blotting.One-way ANO was used for the comparison of measurement data between groups.Results The intraocular pressure of the Scutellarin group[(17.2±1.17)mmHg]was significantly lower than that of the glaucoma group for 7 days after surgery[(22.72±1.91)mmHg,t=8.398,P＜0.05].The retinal thickness of the Scutellarin group[(129.85±7.63)μm]was significantly higher than that of the glaucoma group for 7 days after surgery[(94.53±10.59)μm,t=9.752,P＜0.05].The number of ganglion cells in the Scutellarin group[(23.77±7.88)cells]significantly higher than that in the glaucoma group for 7 days after surgery[(14.92±1.89)cells,t=1.970,P＜0.05].The expression levels of tumor necrosis factor a,interleukin 6,and interleu-kin 1β in the retinal tissues of the Scutellarin group(1.34±0.11,1.39±0.12,1.34±0.05)were sig-nificantly lower than those in the glaucoma group(1.9±0.08,1.78±0.11,1.71±0.08,t=1.540,9.067,13.280,P＜0.05).The apoptosis index in the ret cells of the Scutellarin group[(14.77±1.31)％]was significantly lower than that in glaucoma group for 7 days after surgery[(22.68±6.43)％,t=4.351,P＜.05].The expression levels of p-JNK,p-p38 MAPK,Caspase-3,and bax proteins in the ret tissues of the Scutellarin group(1.38±0.09,0.77±0.08,0.87±0.07,0.72±0.08)were signifi-cantly lower than those in the glaucoma group(1.64±0.06,1.29±0.07,1.56±0.15,1.19±0.08,t=10.760,28.120,22.50,24.840,P＜0.05).Conclusion Scutellarin can inhibit inflammation and reduce ret cell apoptosis,and its protective effect may be related to the inhibition of JNK/p38 MAPK acti-vation.
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