程序性死亡因子配体1表达水平与胰腺癌肿瘤微环境中M2型巨噬细胞比例的相关性研究
The correlation between programmed death ligand 1 expression levels and the proportion of CD163-positive M2 macrophages in the tumor microenvironment of pancreatic cancer
张建民 1刘敬华 1许世峰 2牛纪祥 1张华3
作者信息
- 1. 临沂市人民医院肝胆外科,临沂 276000
- 2. 山东省立医院器官移植肝胆外科,济南 250000
- 3. 临沂市人民医院手术室,临沂 276000
- 折叠
摘要
目的 本研究旨在探讨程序性死亡因子配体1(PD-L1)表达水平与胰腺癌肿瘤微环境中M2型巨噬细胞浸润比例的相关性,以期为胰腺癌的免疫治疗提供新的潜在靶点.方法 收集50例胰腺癌组织样本,采用免疫组织化学染色及流式细胞术检测PD-L1和CD163的表达水平,并应用Pearson相关分析评估两者之间的相关性.结果 胰腺癌组织中PD-L1的表达水平与M2型巨噬细胞的浸润水平呈正相关(r=0.74,P＜0.01),提示PD-L1可能通过增强M2型巨噬细胞的免疫抑制作用实现免疫逃逸,进一步促进肿瘤进展.结论 PD-L1和M2型巨噬细胞在胰腺癌免疫微环境中具有协同作用,PD-L1可能通过增强M2型巨噬细胞的免疫抑制功能,促进肿瘤的免疫逃逸.本研究为联合靶向PD-L1和M2型巨噬细胞的治疗策略提供了理论基础.
Abstract
Objective To investigate the correlation between programmed death ligand 1(PD-L1)expression and the infiltration of CD163-positive M2 macrophages in the tumor microenvironment of pancre-atic cancer,aiming to provide new potential targets for immunotherapy.Methods Pancreatic cancer tissue samples were collected and analyzed for PD-L1 and CD163 expression using immunohistochemistry and flow cytometry.Pearson correlation analysis was used to assess the relationship between these markers.Results The results showed a significant positive correlation between high PD-L1 expression and the infiltration level of M2 macrophages in pancreatic cancer tissues(r=0.74,P＜0.01),suggesting that PD-L1 may facilitate immune evasion by promoting M2 macrophage infiltration.Conclusion High PD-L1 expression may en-hance the immunosuppressive function of M2 macrophages,contributing to immune evasion in pancreatic cancer.These findings support a therapeutic strategy targeting both PD-L1 and M2 macrophages.
关键词
胰腺癌/程序性死亡因子配体1/M2型巨噬细胞/CD163/免疫逃逸Key words
Pancreatic cancer/Programmed death factor ligand 1/M2 macrophages/CD163/Immune escape引用本文复制引用
出版年
2024
NETL
NSTL
万方数据