Expression of nuclear enriched transcript 1 in pediatric glioma tissues and its biological functions
Objective To investigate the expression of nuclear-enriched abundant transcript 1(NEAT1)in pediatric glioma tissues and its biological function.Methods A total of 42 pediatric glioma tissues and adjacent normal tissues collected from March 2021 to March 2024 at Xinxiang central hospital were selected as the research subjects.NEAT1 expression levels in the adjacent normal tissues and glioma tissues were analyzed by quantitative real-time polymerase chain reaction(PCR).Human glioma cell line U87 was divided into si-control group and si-NEAT1 group,respectively transfected with control small in-terfering RNA(siRNA)and NEAT1 siRNA to U87 cells.The proliferation ability of the two groups of cells was analyzed by cell counting kit-8(CCK-8)and BrdU staining.The migration and invasion ability of the two groups of cells was analyzed by Transwell and scratch assays.The effect of NEAT1 on tumor growth was analyzed by nude mouse xenograft experiment.The target genes of NEAT1 were analyzed by bioinformatics and dual luciferase assay.Intergroup metric data comparison was performed by t test.Results The ex-pression level of NEAT1 in peritumoral tissues(0.97±0.10)was significantly lower than that in glioma tissues(1.58±0.19,t=15.040,P＜0.05).The absorbance value of cells in the si-control group(1.86±0.09)was significantly higher than that in the si-NEAT1 group(1.86±0.09,t=15.280,P＜0.05).The BrdU positive rate of cells in the si-control group[(89.07±4.65)％]was significantly higher than that in the si-NEAT1 group[(56.50±5.34)％,t=13.010,P＜0.05].The tumor volume and weight of cells in the si-control group[(845.25±70.35)mm3,(5.88±0.74)g]were significantly higher than those in the si-NEAT1 group[(514.50±80.75)mm3,(2.77±0.32)g,t=8.735,10.900,P＜0.05].The scratch healing rate and migration number of cells in the si-control group[(84.45±4.21)％,(87.17±5.85)cells]were significantly higher than those in the si-NEAT1 group[(56.19±5.75)mm3,(61.23±5.22)cells,t=11.200,9.358,P＜0.05].The proportion of senescent cells in the si-control group[(6.53±1.92)％]was significantly lower than that in the si-NEAT1 group[(31.75±4.46)％,t=14.690,P＜0.05].The expression level of CDKN2A protein in peritumoral tissues(0.79±0.09)was significantly lower than that in glioma tissues(1.42±0.17,t=16.720,P＜0.05).The expression level of CDKN2A protein in the si-control group(1.02±0.11)was significantly higher than that in the si-NEAT1 group(0.67±0.07,t=7.364,P＜0.05).Conclusion NEAT1 is highly expressed in pediatric glioma tissues and participates in the proliferation,migration,and invasion of tumor cells,possibly exerting effects through CDKN2A.
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