摘要
目的:应用浓度为3%的聚桂醇溶液对建立的动物囊性肿瘤模型进行消融治疗，以评估其消融的安全性和有效性并探索该模型模拟胰腺囊性肿瘤的实用性。方法:自体移植法在30只雌性性成熟SD大鼠腹腔内建立子宫内膜异位囊肿模型，饲养4周后成功建模的囊肿随机分为两组分别注射3%聚桂醇溶液或生理盐水，饲养4周后打开腹腔观察囊肿消融情况，取标本进行病理学检查。结果:最终共收集了34个囊肿，形态学方面生理盐水组囊肿术前、术后均保持半球型，而3%聚桂醇溶液组囊肿术前的半球型消融后呈扁平瘢痕状；病理学方面生理盐水组标本上皮层单层柱状上皮细胞几乎无脱落，而3%聚桂醇溶液组标本上皮层单层柱状上皮细胞全部脱落，两组标本固有层均完整。消融术后囊肿无穿孔、腹腔脏器无损伤、大鼠无死亡。统计学分析两组消融有效率表明3%聚桂醇溶液具有良好的消融疗效。结论:3%聚桂醇溶液消融大鼠子宫异位囊肿模型安全且有效，初步表明3%聚桂醇可用于EUS-LA临床治疗。该模型一定程度上可以模拟胰腺囊性肿瘤。
Abstract
Objective:To evaluate the safety and efficacy of 3% lauromacrogol solution for the ablation of animal cystic tumor models, and to explore the practicability of the model for the simulation of pancreatic cystic tumor.Methods:Endometriosis cyst models were established in the abdominal cavity of thirty female mature SD rats by autologous transplantation.After 4 weeks of feeding, the cysts successfully modeled were randomly divided into two groups, injected with 3% lauromacrogol solution or normal saline, respectively.After 4 weeks of feeding, the abdominal cavity was opened to observe the ablation of cysts, and specimens were collected for pathological examination.Results:A total of 34 cysts were collected. In terms of morphology, cysts in the normal saline group remained hemispherical before and after ablation, while cysts in the 3% lauromacrogol solution group showed flat scar after the ablation.In terms of pathology, almost no single layer columnar epithelial cells were ablated in the normal saline group, while all the single layer columnar epithelial cells were ablated in the 3% lauromacrogol solution group, and the lamina propria was intact in both groups. Statistical analysis of the ablation efficiency of the two groups showed that 3% lauromacrogol solution had significant ablation effect.Conclusion:The ablation of endometriosis cyst in rats with 3% lauromacrogol solution is safe and effective, preliminary indicating that 3% lauromacrogol solution can be used for endoscopic ultrasound guided lauromacrogol ablation.This model can simulate pancreatic cystic tumors to some extent.
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