Objective To explore the mechanism of moutan cortex in treating acute renal failure(ARF)complicated by hemorrhagic fever with renal syndrome(HFRS)based on network pharmacology and molecular docking methods.Methods The active ingredients and targets of moutan cortex were screened by TCMSP database.GeneCards database platform was used to predict the disease targets of HFRS and ARF.The intersection of the targets of moutan cortex,HFRS and ARF were determined by Venny 2.1.0.Intersection genes were imported into David database for GO analysis and KEGG pathway enrichment analysis.Cytoscape software was used to construct the"component-target-pathway"network diagram of moutan cortex,HFRS and ARF.PPI network was constructed by STRING database,and topology analysis was carried out by Cytoscape software.AutoDock software was used for molecular docking verification.Results Eleven active ingredients were selected from moutan cortex,acting on 169 targets,109 targets intersections with HFRS and ARF.GO analysis showed that biological processes(BP)were mainly related to drug response,positive regulation of gene expression,negative regulation of apoptotic process and positive regulation of transcription.AGE-RAGE signaling pathway,cancer pathway,lipid and atherosclerosis were the main enrichment pathways in KEGG pathway.Molecular docking results showed that core targets,AKT1,IL6,TNF,TP53 and VEGFA,could form stable structures with quercetin and kaempferol.Conclusion The core targets ofmoutan cortex,such as AKT1,IL6,TNF,TP53 and VEGFA,play roles in the treatment of ARF complicated by HFRS through regulating AGE-RAGE signaling pathway,cancer pathway,lipid and atherosclerosis.
network pharmacologymoutan cortexhemorrhagic fever with renal syndromecomplicationacute renal failure
NETL
NSTL
维普
万方数据
