Objective To understand the distribution of Yersina pestis and conduct the Clustered Regularly Interspaced Short Palindromic Repeat(CRISPR)genotyping in Sanjiangyuan regionto provide scientific basis for Y.pestis identification,tracing,plague detection and control in the region.Methods The DNA was extracted from 310 strains of Y.pestis in the Sanjiangyuan region.The three CRISPR loci of YPa,YPb and YPc were amplified and sequenced by PCR respectively,and then the CRISPR sequences measured were compared with the latest data of CRISPR Dictionary database to identify CRISPR spacer array and genotype Y.pestis in the Sanjiangyuan region.The CRISPR genotypes and isolated host formation of 310 strains of Y.pestis were analyzed statistically.Results Twenty-six spacers including 15 of YPa,8 of YPb and 3 of YPc were observed among 310 isolates of Y.pestis.The 310 strains of Y.pestis were divided into 16 different CRISPR genotypes.G22 and G22-a1′were distributed in the entire Sanjiangyuan region,including Chengduo County,Zhiduo County,Yushu City,Qumalai County,Nangqian County,Zado County,Maduo County,Maqin County,Tongren County,Zeku County,Gonghe County,Tongde County and Guide County.G24 distributed in Zeku County,Maduo County,Nangqian County,Chengduo County;G26-a1′type is mainly distributed in Gonghe County,Xinghai County,Guide County,Maduo County,Chengduo County,Zadoi County.The 16 genotypes were grouped into seven CRISPR groups:Cb4,Cb4′,Ca7,Ca7′,Ca8,Ca35′ and Cc3′.Conclusion The CRISPR have diversity genotypes of Y.pestis in Sanjiangyuan region,and the genotypes of different foci have significant distribution characteristics,and the genotypes of different strains are different.
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