Objective To investigate the basic situation of pathogens carried by small mammal in Qingdao,and to provide theoretical basis for the prevention and control of rodent diseases.Methods Nucleic acid samples of liver,spleen,kidney and lung tissues of small mammal were collected from the West Coast New District,Jimo District,Jiaozhou City,Pingdu City and Laixi City during 2021-2023.The qPCR was used to detect pathogenic Leptospirosis and Bartonella,and the RT-qPCR was used to detect Hantavirus and novel Bunyavirus.And the population composition ratio and the infection rate of pathogens were analyzed statistically.Results A total of 624 small mammals were captured,among which Rattus norvegicus accounted for 43.11%,Mus musculus accounted for 36.86%,Apodemus agrarius accounted for 13.94%,Sorex spp.accounted for 6.09%.The pathogen detection results showed that the infection rate of Hantavirus in mouse borne pathogens was 3.37%.The infection rate of pathogenic Leptospira is 0.96%,and Bartonella is 1.12%.No novel Bunyavirus was detected.Three kinds of pathogens were detected in both Rattus norvegicus and Mus musculus,and the highest infection rate was Rattus norvegicus.The highest infection rate of rodent pathogens in farmland was 8.20%.Conclusion There are cases of rodent pathogens such as Hantavirus,Bartonella,and pathogenic Leptospira in Qingdao.It is necessary to strengthen the monitoring and prevention of rodent borne pathogens in rural areas to reduce the risk of rodent borne infections in the population.
small mammalHantaviruspathogenic LeptospiraBartonella