Objective To understand the nesting structure and biological learning characteristics of Pericapritermes nitobei(Shiraki),and to grasp the physical and chemical characteristics of the worker intestine of worker termite,in order to provide essential basic information for the production and utilization of this termite.Methods Field investigation and laboratory observation were combined to clarify the nest structure and biological habit of Pericapritermes nitobei.Intestinal anatomy and biochemical assay were used to analyze different parts of intestinal structure and physiological characteristics of Pericapritermes nitobei.Results The Pericapritermes nitobei feed humus in soil.The digestive tract of worker termites contains ammonia and nitrate,but nitrite content is very low.The rectum(P5)had the highest levels of ammonia and nitrate,followed by the tubular colon(P4).From the crop(C)to the ileum(P1),the pH value gradually increased,reached the highest value in the ileum,and then gradually decreased to weakly acidic.On the contrary,the REDOX potential decreased first and then increased from the wordy sac(C)to the posterior intestine,and the ileum(P1)segment was the lowest.There was no significant change in the hydrogen content in the whole digestive tract.Conclusion The intestinal physiological characteristics of its workers are unique.Therefore,it is necessary to consider the different physiological characteristics of Pericapritermes nitobei in biodegradation of agricultural and forestry waste.