Analysis of voltage-gated sodium channel gene mutations of Aedes albopictus populations in park and rurual habitat in Wuhan City
Objective To investigate the voltage-gated sodium channel(VGSC)gene mutations of Aedes albopictus in park and rural habitat in Wuhan City.Methods Larvae and pupae of Ae.albopictus were collected from 2 parks and 2 villages in Wuhan City during July to October 2022 and then raised to adult mosquitoes in the laboratory.Genomic DNA was extracted from individual mosquito and the partial sequence of VGSC gene fragments were amplified and sequenced to detect the mutation.The distribution of mutant alleles at 3 loci of VGSC gene were compared between different Ae.albopictus populaitons by Chi-square test.Results A total of 104 Ae.albopictus from park habitat and 43 Ae.albopictus from rural habitat were analyzed.Mutations were detected at the 1016,1532 and 1534 loci of VGSC gene in park population,where joint mutant alleles at two of the three loci(i.e.1016+1532,1532+1534 loci)were found.Anyway,mutations were detected at the 1016 and 1534 loci but not at 1532 loci of VGSC gene in rural population,where no joint mutation was observed.The rate of mutant allele TCC/S and TGC/C at 1534 loci was significant higher in the park population than in rural population,with P＜0.001,while no significant difference was found in the rate of mutant allele GGA/G at 1016 loci or mutant allele ACC/T at 1532 loci between the two Ae.albopictus field populations,with P=0.160 and P=0.263,respectively.Conclusion The rate of VGSC gene mutation was various between the park Ae.albopictus population and rural Ae.albopictus population.It is suggested to monitor the VGSC gene mutation to better understand the mechanism of rapidly developed pyrethroid insecticides resistance in Ae.albopictus.
万方数据
维普
