Detection of rpsL mutations in streptomycin resistant gene of Yersinia pestis from different natural foci in Gansu Province
Objective To detect the mutations of rpsL gene in strains of Yersinia pestis from different natural foci in Gansu Province,so as to provide basis for clinical medication of the plague patients and monitoring on drug resistance of Yersinia pestis.Methods DNA was extracted from 376 strains of Yersinia pestis isolated from different natural foci of Gansu province from 1962 to 2022.PCR primers and TaqMan-MGB fluorescent probes were designed according to the rpsL mutations of Yersinia pestis with the streptomycin resistant.The mutation sites of the rpsL gene were analyzed by direct sequencing of PCR products,and the 128 base mutations in the rpsL gene of Yersinia pestis from different foci of Gansu Province were detected by Real-time PCR.Results It was found that the entire rpsL gene sequence of Yersinia pestis from different types of plague foci in Gansu Province did not change through the sequence comparison analysis of 376 rpsL gene,and the rpsL gene base sequence of Yersinia pestis from different years was the same,which confirmed that the rpsL gene sequence of Yersinia pestis in Gansu Province was conserved.FAM test results of 376 strains of Yersinia pestis from different natural foci in Gansu Province were positive,corresponding to rpsL(128:A),△Rn＞3 000 000;The VIC test results of all strains were negative,corresponding to rpsL(128:G),△ Rn＜100 000.Conclusion It is recommended to monitor the drug resistance of Yersinia pestis in the Himalayan Marmota foci of Gansu Province where the animal plage continues to spread,so as to find out the mutations of rpsL gene in Yersinia pestis in time,and provide basis for the diagnosis and treatment of human plague.
万方数据
维普
