摘要
探究重楼皂苷Ⅶ(PPⅦ)对弥漫大B细胞淋巴瘤(DLBCL)细胞株U2932和SUDHL-4的增殖、凋亡和细胞周期的影响.实验将DLBCL细胞株分为对照组和PPⅦ组,并使用MTT法、流式细胞术和Western blot法进行实验.结果显示,与对照组相比,PPⅦ显著抑制了 U2932和SUDHL-4细胞的增殖(P＜0.05).细胞凋亡实验表明,0.5和1 μmol/L的PPⅦ处理使得两种细胞的凋亡率显著增加(P＜0.05),并且凋亡相关蛋白的表达上调,而Bcl-2蛋白水平显著降低(P＜0.05).细胞周期实验显示,PPⅦ处理使得G0/G1期细胞增加(P＜0.05),G2/M期细胞减少(P＜0.05),且Cyclin D1、CDK4、CDK6和Survivin蛋白的表达量明显下调(P＜0.05).综上所述,PPⅦ通过抑制DLBCL细胞的增殖、促进细胞凋亡以及阻滞细胞于G0/G1期,发挥了抗淋巴瘤的作用.
Abstract
The aim of this study was to investigate the effects of polyphyllin Ⅶ(PP Ⅶ)on proliferation,apoptosis,and cell cycle of diffuse large B-cell lymphoma(PLBCL)cell lines U2932 and SUDHL-4.The DLBCL cell lines were divided into a control group and a PP Ⅶ group,and experiments were conducted using MTT assay,flow cytometry,and Western blotting.Results showed that compared with the control group,PPⅦ significantly inhibited the proliferation of U2932 and SUDHL-4 cells(P＜0.05).Apoptosis assays demonstrated that treatment with 0.50 and 1.00 μmol/L PP Ⅶ significantly increased the apoptosis rates of both cell lines(P＜0.05),upregulated apoptosis-related proteins,and downregulated Bcl-2 protein level(P＜0.05).Cell cycle analysis revealed that PPⅦ treatment led to an increase in G0/G1-phase cells(P＜0.05)and a decrease in G2/M-phase cells(P＜0.05),significantly downregulated cyclin D1,CDK4,CDK6,and survivin protein expression(P＜0.05).In conclusion,PP Ⅶexerted anti-lymphoma effects by inhibiting proliferation,promoting apoptosis,and inducing G0/G1 phase arrest in DLBCL cells.
基金项目
山东省自然科学基金青年基金(ZR2022QH168)
山东省自然科学基金面上项目(ZR2022MH058)
江苏省新药研究与临床药学重点实验室开放基金(XZSYS-KF2021033)
临沂市重点研发计划(医学类)(2022YX0117)
NETL
NSTL
万方数据