The aim of this study was to investigate the effects of polyphyllin Ⅶ(PP Ⅶ)on proliferation,apoptosis,and cell cycle of diffuse large B-cell lymphoma(PLBCL)cell lines U2932 and SUDHL-4.The DLBCL cell lines were divided into a control group and a PP Ⅶ group,and experiments were conducted using MTT assay,flow cytometry,and Western blotting.Results showed that compared with the control group,PPⅦ significantly inhibited the proliferation of U2932 and SUDHL-4 cells(P＜0.05).Apoptosis assays demonstrated that treatment with 0.50 and 1.00 μmol/L PP Ⅶ significantly increased the apoptosis rates of both cell lines(P＜0.05),upregulated apoptosis-related proteins,and downregulated Bcl-2 protein level(P＜0.05).Cell cycle analysis revealed that PPⅦ treatment led to an increase in G0/G1-phase cells(P＜0.05)and a decrease in G2/M-phase cells(P＜0.05),significantly downregulated cyclin D1,CDK4,CDK6,and survivin protein expression(P＜0.05).In conclusion,PP Ⅶexerted anti-lymphoma effects by inhibiting proliferation,promoting apoptosis,and inducing G0/G1 phase arrest in DLBCL cells.
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