Bone marrow biopsy is one of the important means of hematopathological diagnosis,which has decisive diagnostic significance for various benign and malignant lymphohematopoietic system diseases.Its diagnostic value includes morphological observation,immunohistochemistry,genetics,and mo-lecular biology testing.Owing to the unique nature of bone marrow biopsy,decalcification is an essential step in the pre-treatment process.Its purpose is to remove calcium from bone tissue,preserve intact colla-gen fiber components,facilitate tissue sectioning,and prevent tissue detachment during staining.If bone marrow biopsy lacks sufficient decalcification,preparing a section is difficult.Conversely,if decalcification is excessive,it can seriously disrupt tissue antigen activity.Therefore,a decalcification method with high de-calcification efficiency and mild antigen damage is essential for bone marrow biopsy.This article introduc-es a bone marrow biopsy tissue decalcification method with high efficiency and less antigen loss:decalcifi-cation is performed at room temperature with 12％formic acid and 8％hydrochloric acid decalcification so-lution on a shaker.
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