Mass spectrometry is a spectroscopic technique for detecting the molecular weight of substances based on mass spectrometry equipment.Many types of mass spectrometry with different functions are widely used in scientific research and application technology development in various disciplines.In recent years,mass spectrometry has shown great potential in nucleic acid detection.In particular,matrix-assisted laser desorption/ionization time of flight mass spectrometry has become a research hotspot due to its velocity,high throughput,and accuracy.The nucleic acid research by mass spectrometry is highlighted in single nucleotide polymorphism,gene mutation,DNA methylation analysis,and DNA copy number variations.This article reviews the research and application of mass spectrometry in nucleic acid detection and analysis to provide a reference for the development of new detection technology for nucleic acid based on mass spectrometry.
关键词
质谱/DNA甲基化/单核苷酸多态性/DNA拷贝数变异/多重PCR-质谱微测序
Key words
Mass spectrometry/DNA methylation/Single nucleotide polymorphism/DNA copy number variations/Multiplex polymerase chain reaction-Mass spectrometry mini-sequencing
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