Association between long-term exposure to ambient ozone and sperm quality in Shandong Province
曲一楠 1韩文凯 2宋晓辉 2赵琦 1马伟 1王丽 2张振伟
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作者信息
1. 山东大学齐鲁医学院公共卫生学院流行病学系，济南 250012
2. 山东大学妇儿与生殖健康研究院（山东大学附属生殖医院），济南 250012
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摘要
目的 探讨山东省大气O3长期暴露与精子质量的关联。 方法 于2014年1月1日至2019年12月31日通过山东大学附属生殖医院人类精子库招募健康捐精志愿者，共计对2 971名健康志愿者的37 977份捐精数据进行了分析；年均O3浓度（0.01°×0.01°）按家庭住址匹配。采用多元混合效应模型分析前1年平均O3暴露浓度与精子质量的暴露-反应关系，以每位捐精者作为随机截距，估计O3增加每10 μg/m3相关精液质量参数的百分比变化，采用分层分析评估个体特征对O3与精液质量的修饰效应。 结果 精液采集前1年平均O3浓度为（107.09±7.50）μg/m3，O3暴露与精子浓度（-3.12%，95%CI：-4.55%，-1.67%）、精子总数（-5.21%，95%CI：-7.28%，-3.09%）、精子活动率（-1.49%，95%CI：-2.37%，-0.61%）、前向运动精子比率（-2.53%，95%CI：-3.78%，-1.26%）、活动精子总数（-5.82%，95%CI：-8.17%，-3.41%）和前向运动精子总数（-6.22%，95%CI：-8.73%，-3.64%）下降有强关联。30岁及以上、肥胖且学历较低男性的精子质量可能更容易受到O3的影响，但差异无统计学意义（P>0.05）。 结论 山东省大气O3长期暴露与精子质量下降有关联。 Objective To evaluate the association between long-term exposure to ambient ozone (O3) and sperm quality. Methods From January 1, 2014, to December 31, 2019, healthy sperm donors were recruited through the Human Sperm Bank of Shandong University Affiliated Reproductive Hospital. A total of 37 977 sperm donation data from 2 971 healthy volunteers were analyzed. The average annual O3 concentration (0.01°× 0.01°) was matched according to household address. A multivariate mixed-effect model was used to analyze the exposure-response relationship between the average O3 exposure concentration and sperm quality in the previous year, with each donor as a random intercept. All results were presented as % changes with 95% confidence intervals (CIs) for all sperm parameters associated with 10 μg/m3 increases in O3. The effects of individual characteristics on the association between O3 and sperm quality were evaluated by stratified analysis. Results The average O3 concentration in the year before semen collection was (107.09±7.50) μg/m3. Each 10 μg/m3 increase in O3 was associated with declined sperm concentration (-3.12%, 95%CI:-4.55%, -1.67%), total sperm count (-5.21%, 95%CI:-7.28%, -3.09%), total sperm motility (-1.49%, 95%CI:-2.37%, -0.61%), progressive motility (-2.53%, 95%CI:-3.78%, -1.26%), total motile sperm count (-5.82%, 95%CI:-8.17%, -3.41%), and progressively motile sperm count (-6.22%, 95%CI:-8.73%, -3.64%). Men aged 30 and above, obese, and with lower education levels might be more susceptible to the influence of O3 on sperm quality, but the difference was not statistically significant (P>0.05). Conclusion Long-term exposure to O3 in Shandong Province is associated with a decrease in sperm quality.
Abstract
Objective To evaluate the association between long-term exposure to ambient ozone (O3) and sperm quality. Methods From January 1, 2014, to December 31, 2019, healthy sperm donors were recruited through the Human Sperm Bank of Shandong University Affiliated Reproductive Hospital. A total of 37 977 sperm donation data from 2 971 healthy volunteers were analyzed. The average annual O3 concentration (0.01°× 0.01°) was matched according to household address. A multivariate mixed-effect model was used to analyze the exposure-response relationship between the average O3 exposure concentration and sperm quality in the previous year, with each donor as a random intercept. All results were presented as % changes with 95% confidence intervals (CIs) for all sperm parameters associated with 10 μg/m3 increases in O3. The effects of individual characteristics on the association between O3 and sperm quality were evaluated by stratified analysis. Results The average O3 concentration in the year before semen collection was (107.09±7.50) μg/m3. Each 10 μg/m3 increase in O3 was associated with declined sperm concentration (-3.12%, 95%CI:-4.55%, -1.67%), total sperm count (-5.21%, 95%CI:-7.28%, -3.09%), total sperm motility (-1.49%, 95%CI:-2.37%, -0.61%), progressive motility (-2.53%, 95%CI:-3.78%, -1.26%), total motile sperm count (-5.82%, 95%CI:-8.17%, -3.41%), and progressively motile sperm count (-6.22%, 95%CI:-8.73%, -3.64%). Men aged 30 and above, obese, and with lower education levels might be more susceptible to the influence of O3 on sperm quality, but the difference was not statistically significant (P>0.05). Conclusion Long-term exposure to O3 in Shandong Province is associated with a decrease in sperm quality.
关键词
臭氧/精子/队列研究/长期暴露/敏感人群
Key words
Ozone/Spermatozoa/Cohort studies/Long-term exposure/Sensitive population
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