中华炎性肠病杂志(中英文)2024，Vol.8Issue(6) ：434-439.DOI:10.3760/cma.j.cn101480-20230914-00019

肠上皮C1GalT1蛋白与溃疡性结肠炎疾病活动度的相关性研究

Study of correlation between C1GalT1 protein and disease activity in ulcerative colitis

汪泽慧 姜蔚 鲁逸涵 李丹 张航 张军
中华炎性肠病杂志(中英文)2024，Vol.8Issue(6) ：434-439.DOI:10.3760/cma.j.cn101480-20230914-00019
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肠上皮C1GalT1蛋白与溃疡性结肠炎疾病活动度的相关性研究

Study of correlation between C1GalT1 protein and disease activity in ulcerative colitis

汪泽慧 1姜蔚 1鲁逸涵 1李丹 1张航 1张军1
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作者信息

  • 1. 南京医科大学附属南京医院消化内科,南京 210006
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摘要

目的 分析溃疡性结肠炎(UC)患者肠上皮核心1β1,3-半乳糖基转移酶(C1GalT1)的表达水平与疾病活动度的关系,并探讨其用于预测UC活动度的可能性.方法 采用回顾性病例对照研究方法.收集2013年11月至2023年2月南京医科大学附属南京医院消化内科收治的77例UC患者(UC组)及18例同期接受体检的健康对照者(正常对照组)资料.使用免疫组化方法测定两组肠上皮C1GalT1蛋白的表达情况.采用改良Mayo评分评估UC的疾病活动度,其中评分≤2分且单项＜2分为缓解期,3～5分为轻度活动期,6～10分为中度活动期,≥11分为重度活动期.统计分析C1GalT1蛋白在UC组和正常对照组以及不同活动度UC患者间的表达差异,采用Spearman检验分析C1GalT1蛋白与活动度、C反应蛋白(CRP)和红细胞沉降率(ESR)的相关性,通过受试者工作特征(ROC)曲线评估C1GalT1蛋白对UC活动度的诊断效能.结果 UC组中,男33例,女44例;年龄(48.22±1.81)岁;病程4.0(0.5,10.0)年;缓解期9例,轻度活动期15例,中度活动期34例,重度活动期19例.正常对照组中,男7例,女11例;年龄(53.44±3.35)岁.两组年龄、性别差异均无统计学意义(均P＞0.05).UC患者C1GalT1蛋白表达低于正常对照组(P＜0.000 1);且随着UC活动度增高,蛋白表达逐渐降低,差异均具有统计学意义(均P＜0.05)o C1GalT1蛋白与UC活动度(r=-0.716 1,P＜0.000 1)、CRP(r=-0.334,P＜0.01)、ESR(r=-0.383,P＜0.001)均呈负相关.在正常-UC的诊断效能上,C1GalT1蛋白的截断值为33.16％时,灵敏度为94.4％,特异性为98.7％,曲线下面积(AUC)为0.994;在轻度-中度活动的诊断效能上,截断值为29.96％,灵敏度为100％,特异性为91.2％,AUC为0.978;在中度-重度活动的诊断效能上,截断值为26.9％,灵敏度为79.4％,特异性为78.9％,AUC为0.805.结论 糖基转移酶C1GalT1蛋白的表达与UC患者活动度显著相关,可能成为UC活动度的标志物.

Abstract

Objective To analyze the relationship between the expression level of core 1 β1,3-galactosyltransferase(C1GalT1)and activity of ulcerative colitis(UC),and to explore its potential use in predicting UC activity.Methods A retrospective case-control study was constructed.The data of 77 UC patients(UC group)and 18 healthy controls(normal contro group)were collected from November 2013 to February 2023 at the Department of Gastroenterology,Nanjing Hospital of Nanjing Medical University.Immunohistochemistry was used to measure the expression of C1GalT1 protein in the intestinal epithelium of both groups.The modified Mayo score was used to assess UC disease activity,with scores ≤ 2 and individual items＜2 considered as remission,scores of 3-5 as mild activity,scores of 6-10 as moderate activity,and scores ≥ 11 as severe activity.Statistical analysis was conducted to compare the differences in C1GalT1 protein expression between the UC group and the normal control group,as well as among UC patients with different activities.The Spearman test was used to analyze the correlations of C1GalT1 protein with disease activity,C-reactive protein(CRP),and erythrocyte sedimentation rate(ESR).The diagnostic efficacy of C1GalT1 protein for UC activity was evaluated using receiver operating characteristic(ROC)curves.Results In the UC group,there were 33 males and 44 females,with an average age of(48.22±1.81)years and a disease duration of 4.0(0.5,10.0)years.The group included 9 patients in remission,15 with mild activity,34 with moderate activity,and 19 with severe activity.In the normal control group,there were 7 males and 11 females,with an average age of(53.44±3.35)years.There were no statistically significant differences in age and gender between the two groups(both P＞0.05).The expression of C1GalT1 protein in UC patients was lower than that in the normal control group(P＜0.000 1).Additionally,as UC activity increased,C1GalT1 protein expression gradually decreased,with all differences being statistically significant(all P＜0.05).C1GalT1 protein was negatively correlated with UC activity(r=-0.716 1,P＜0.000 1),CRP(r=-0.334,P＜0.01),and ESR(r=-0.383,P＜0.001).Regarding the diagnostic efficacy of distinguishing between the normal control and UC groups,when the cutoff value for C1GalT1 protein was set at 33.16％,the sensitivity was 94.4％,the specificity was 98.7％,and the area under curve(AUC)was 0.994.For diagnosing mild to moderate activity,when the cutoff value was 29.96％,the sensitivity was 100％,the specificity was 91.2％,and the AUC was 0.978.For diagnosing moderate to severe activity,when the cutoff value was 26.9％,the sensitivity was 79.4％,the specificity was 78.9％,and the AUC was 0.805.Conclusion The expression of glycosyltransferase C1GalT1 protein is significantly correlated with the activity of UC patients and may serve as a marker for UC activity.

关键词

溃疡性结肠炎/核心1β1,3-半乳糖基转移酶/疾病活动度/O-聚糖/相关

Key words

Ulcerative colitis/Core 1 β1,3-galactosyltransferase/Disease activity/O-glycans/Correlation

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出版年

2024
中华炎性肠病杂志(中英文)

中华炎性肠病杂志(中英文)

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