目的 分析视神经炎患者血清特征性代谢物。 方法 病例对照研究。收集2021年1月至2022年1月北京同仁医院神经内科确诊的视神经炎患者血清样本，同时收集性别、年龄匹配的健康体检者血清样本作为对照组。其中，视神经炎组9例，女性5例，男性4例，年龄（35.8±12.9）岁，对照组9例，女性5例，男性4例，年龄（32.6±8.6）岁。采用液相色谱质谱技术分析视神经炎患者、健康人群血清中的代谢物。选择主成分分析（PCA）及正交偏最小二乘法分析（OPLS-DA）的多元统计分析方法，采用OPLS-DA模型的变量投影重要性（VIP）值并结合t检验的P值筛选差异代谢物。 结果 鉴定出37种特征代谢物，VIP>1且P<0.05的差异代谢物共有4种，3种为磷脂类物质，分别为LysoPC（P-16∶0）、LysoPC（16∶0）、LysoPC（P-18∶0），1种为苏氨酸，4种代谢物含量均下调，4种代谢物受试者工作曲线下面积分别为0.951、0.889、0.963和0.944。 结论 基于代谢组学分析，视神经炎患者血清中的一些代谢物发生了变化，可为视神经炎血清标志物的筛选提供实验基础。 Objective To investigate the serum metabolites and their metabolic characteristics of patients with optic neuritis. Methods . Case-control study. From January 2021 to January 2022, 9 serum specimens of diagnosed patients with optic neuritis were collected in Department of Neurology from Beijing Tongren Hospital and 9 healthy subjects as the control. Among them, there were 5 females and 4 males in the optic neuritis group, aged (35.8±12.9) years there were 5 females and 4 males in the healthy control group, aged (32.6±8.6) years. Liquid chromatography-mass spectrometry was used to detect metabolites in serum of healthy control and patients with optic neuritis. The principal component analysis (PCA) and orthogonal partial least-squares discriminination analysis (OPLS-DA) were used to analyze the differential metabolites The variable importance projection value of OPLS-DA model and the P value of t-test was applied to find the different metabolites. Results Thirty-seven metabolites were finally identified from serum samples. Four metabolites with variable important in projection (VIP) values larger than 1 and P values less than 0.05 were teased out, three metabolites, LysoPC (P-16∶0), LysoPC (16∶0), LysoPC (P-18∶0) belonge to phospholipid and one metabolite was L-Threonine, they were all down-regulated. The area under curve were 0.951, 0.889, 0.963 and 0.944, respectively. Conclusion Based on metabonomic analysis, some metabolites in serum have changed, which can provide basis for biomarkers screening of optic neuritis.
Preliminary analysis of serum metabolomics in patients with optic neuritis based on liquid chromatography-mass spectrometry
Objective To investigate the serum metabolites and their metabolic characteristics of patients with optic neuritis. Methods . Case-control study. From January 2021 to January 2022, 9 serum specimens of diagnosed patients with optic neuritis were collected in Department of Neurology from Beijing Tongren Hospital and 9 healthy subjects as the control. Among them, there were 5 females and 4 males in the optic neuritis group, aged (35.8±12.9) years there were 5 females and 4 males in the healthy control group, aged (32.6±8.6) years. Liquid chromatography-mass spectrometry was used to detect metabolites in serum of healthy control and patients with optic neuritis. The principal component analysis (PCA) and orthogonal partial least-squares discriminination analysis (OPLS-DA) were used to analyze the differential metabolites The variable importance projection value of OPLS-DA model and the P value of t-test was applied to find the different metabolites. Results Thirty-seven metabolites were finally identified from serum samples. Four metabolites with variable important in projection (VIP) values larger than 1 and P values less than 0.05 were teased out, three metabolites, LysoPC (P-16∶0), LysoPC (16∶0), LysoPC (P-18∶0) belonge to phospholipid and one metabolite was L-Threonine, they were all down-regulated. The area under curve were 0.951, 0.889, 0.963 and 0.944, respectively. Conclusion Based on metabonomic analysis, some metabolites in serum have changed, which can provide basis for biomarkers screening of optic neuritis.
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