Objective To establish the reference intervals of reticulocyte parameters for children in the Beijing area.Methods 1 766(856 males and 910 females)healthy children aged from 1 to 18 years old in the Beijing area were studied,the infant group(≥1-3 years old)included 146 participants;the preschool group(＞3-6 years old)had 449 participants;the school age group(＞6-13 years old)had 646 participants and the adolescent group(＞13-18 years old)had 525 participants.Seven parameters were measured in venous blood by SySmex XN-20(A1)automatic blood cell analyzer,which included reticulocyte percentage(RET％),absolute reticulocyte count(RET#),low fluorescence reticulocyte(LFR),medium fluorescence reticulocyte(MFR),high fluorescence reticulocyte(HFR),immature reticulocyte fraction(IRF)and reticulocyte hemoglobin equivalent(RET-He).After the test results were collected,the reference intervals were established according to the percentile(P25,P97.5).As the reference intervals were established,venous blood samples from 109 healthy children in Beijing were collected to verify the reference intervals according to WS/T 402-2012"Define and determine the reference intervals in clinical laboratory".Results The reference interval of 7 Reticulocyte parameters for children aged 1-18 years without age and sex grouping,reference interval(RET％):0.74％-2.06％;absolute reticulocyte count(RET#):(34.5-101.5)x109/L;low fluorescence reticulocyte(LFR):86.6％-96.5％;medium fluorescence reticulocyte(MFR):3.2％-11.5％;high fluorescence reticulocyte(HFR):0％-2.1％;immature reticulocyte fraction(IRF):3.5％-13.4％;reticulocyte hemoglobin equivalent(RET-He):28.5-34.2 pg.Over 90％test results of samples from the 109 children ranged within the reference ranges.Conclusion This study established convincible reference intervals of seven reticulocyte parameters for healthy children aged from 1 to 18 in the Beijing area was established,and reference interval verification passed.
维普
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