Research of multiparameter flow cytometry in detecting non-neoplastic abnormal cell phenotypes in human bone marrow
Objective To investigate the role of multiparameter flow cytometry(MFC)in detecting non-neoplastic abnormal phenotypes,including monoclonal gammopathy of undetermined significance(MGUS),monoclonal B-cell lymphocytosis(MBL),Fcγ receptor Ⅲ b(FcγRⅢ B),and CD36 deficiencies.Methods A retrospective and observational study was conducted on a total of 24 864 patients who underwent one-step screening for leukemia/lymphoma at Hebei Yanda Ludaopei Hospital from January 3,2020,to March 31,2024.Clinical data and MFC results of the patients were collected.Patients were grouped by age,group of＜45 years(11 495 cases),group of 45-＜60 years(5 322 cases),group of ≥60 years(7 081 cases)and disease nature,benign group(8 336 cases),malignant group(15 562),and other diseases group(966 cases).The incidence of non-neoplastic abnormal phenotypes was analyzed across groups.Results The incidence rates of MGUS,MBL,FcγRⅢB,and CD36 deficiencies were 0.072％(18/24 864),0.511％(127/24 864),0.221％(55/24 864),and 0.004％(1/24 864),respectively.MBL incidence was lower in the malignant group than in the benign group(P＜0.001),while MGUS incidence was higher in the malignant group than in the benign group(P=0.034).The incidence rate of MBL among subtypes within the benign group varied significantly(P＜0.001),with the highest incidence in patients with pancytopenia at 2.72％(48/1 765).In the malignant group,the incidence of MBL differed significantly across various disease types(P＜0.001),among which MDS/MPN exhibited the highest incidence at 1.95％(3/154)and 1.30％(2/154).Conclusion Utility of multiparameter flow cytometry method for one-step screening of leukemia and lymphoma shows variety in detecting non-neoplastic abnormal phenotypes,facilitating the identification of diseases.
Flow cytometryMonoclonal gammopathy of undetermined significanceMonoclonal B-cell lymphocytosisFcγRⅢ B deficiency
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维普
