摘要
目的 分析多参数流式细胞术(MFC)检测骨髓标本中非肿瘤性异常表型相关疾病,包括意义不明的单克隆免疫球蛋白血症(MGUS)、单克隆B细胞增多症(MBL)、Fcγ受体Ⅲ类B(FcγRⅢB)缺失、CD36缺失等的表型特征.方法 回顾性观察性研究.选取2020年1月3日至2024年3月31日于河北燕达陆道培医院进行骨髓白血病/淋巴瘤一步法MFC筛查肿瘤性疾病的24 864例患者.收集患者一般临床资料以及MFC结果.根据年龄分为＜45岁组(11 495例)、45～＜60岁组(5 322例)、≥60岁组(7 081例);按疾病性质分为良性疾病组(8 336例)、恶性疾病组(15 562例)、其他组(966例).分析各组骨髓标本中非肿瘤性异常表型的发生情况.结果 MGUS、MBL、FcγRⅢB缺失、CD36缺失的发生率分别为 0.072％(18/24 864)、0.511％(127/24 864)、0.221％(55/24 864)、0.004％(1/24 864).恶性疾病组MBL的发病率低于良性疾病组(P＜0.001),MGUS的发病率高于良性疾病组(P=0.034).良性疾病组中各疾病类型的MBL发生率比较,差异有统计学意义(P＜0.001),其中全血细胞减少患者中MBL发生率最高为2.72％(48/1 765);恶性疾病组中,各疾病类型的MBL、MGUS发生率比较,差异均有统计学意义(P均＜0.001),其中骨髓增生异常综合征/骨髓增殖性疾病患者发生率均最高,分别为1.95％(3/154)、1.30％(2/154).结论 应用MFC进行白血病/淋巴瘤一步法检测的非肿瘤性异常细胞表型特征存在一定差异,对疾病的筛查具有一定的借鉴意义.
Abstract
Objective To investigate the role of multiparameter flow cytometry(MFC)in detecting non-neoplastic abnormal phenotypes,including monoclonal gammopathy of undetermined significance(MGUS),monoclonal B-cell lymphocytosis(MBL),Fcγ receptor Ⅲ b(FcγRⅢ B),and CD36 deficiencies.Methods A retrospective and observational study was conducted on a total of 24 864 patients who underwent one-step screening for leukemia/lymphoma at Hebei Yanda Ludaopei Hospital from January 3,2020,to March 31,2024.Clinical data and MFC results of the patients were collected.Patients were grouped by age,group of＜45 years(11 495 cases),group of 45-＜60 years(5 322 cases),group of ≥60 years(7 081 cases)and disease nature,benign group(8 336 cases),malignant group(15 562),and other diseases group(966 cases).The incidence of non-neoplastic abnormal phenotypes was analyzed across groups.Results The incidence rates of MGUS,MBL,FcγRⅢB,and CD36 deficiencies were 0.072％(18/24 864),0.511％(127/24 864),0.221％(55/24 864),and 0.004％(1/24 864),respectively.MBL incidence was lower in the malignant group than in the benign group(P＜0.001),while MGUS incidence was higher in the malignant group than in the benign group(P=0.034).The incidence rate of MBL among subtypes within the benign group varied significantly(P＜0.001),with the highest incidence in patients with pancytopenia at 2.72％(48/1 765).In the malignant group,the incidence of MBL differed significantly across various disease types(P＜0.001),among which MDS/MPN exhibited the highest incidence at 1.95％(3/154)and 1.30％(2/154).Conclusion Utility of multiparameter flow cytometry method for one-step screening of leukemia and lymphoma shows variety in detecting non-neoplastic abnormal phenotypes,facilitating the identification of diseases.
维普
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