Expert Consensus on isolation and detection technology of extracellular vesicles
Extracellular vesicle(EV)serves as a crucial mediator of intercellular information exchange,playinga pivotal role in human pathophysiological processes and offering immense potential in the realm of liquid biopsy.However,a diverse array of EV isolation and detection techniques exist,each possessing its own strengths and limitations.Hence,the establishment of an expert consensus on EV isolation and detection techniques holds significant value in advancing the translational and clinical application of EV research.This consensus,jointly authored by the Chinese Society of Extracellular Vesicles of the Chinese Research Hospital Association and the Molecular Diagnostics Group of the Chinese Society of Laboratory Medicine,combines the insights and experiences of experts spanning diverse related fields,encompassinglaboratory medicine,basic medicine,clinical medicine,analytical chemistry,biology,and materials science,among others.It elaborates a comprehensive overview of commonly employed EV isolation and detection techniques,along with their applications in diverse scenarios.Furthermore,it identifies the key technological considerations for the clinical translation of EV applications.