摘要
细胞外囊泡是细胞间传递信息的重要载体,在人体病理生理过程中发挥着重要作用,在液体活检领域也有巨大潜力.然而,目前细胞外囊泡分离与检测技术多样,各有优缺点.因此,建立细胞外囊泡分离与检测技术专家共识,对于推动EV的研究转化与临床应用具有重要价值.本共识由中国研究型医院学会细胞外囊泡研究与应用专业委员会和中华医学会检验医学分会分子诊断学组共同编写,汇聚了来自检验医学、基础医学、临床医学、分析化学、生物学和材料科学等多个相关领域专家的观点和经验,详细阐述了常用细胞外囊泡分离与检测技术及其不同应用场景,并进一步指明了细胞外囊泡临床应用转化的关键技术要点.
Abstract
Extracellular vesicle(EV)serves as a crucial mediator of intercellular information exchange,playinga pivotal role in human pathophysiological processes and offering immense potential in the realm of liquid biopsy.However,a diverse array of EV isolation and detection techniques exist,each possessing its own strengths and limitations.Hence,the establishment of an expert consensus on EV isolation and detection techniques holds significant value in advancing the translational and clinical application of EV research.This consensus,jointly authored by the Chinese Society of Extracellular Vesicles of the Chinese Research Hospital Association and the Molecular Diagnostics Group of the Chinese Society of Laboratory Medicine,combines the insights and experiences of experts spanning diverse related fields,encompassinglaboratory medicine,basic medicine,clinical medicine,analytical chemistry,biology,and materials science,among others.It elaborates a comprehensive overview of commonly employed EV isolation and detection techniques,along with their applications in diverse scenarios.Furthermore,it identifies the key technological considerations for the clinical translation of EV applications.
维普
万方数据