Construction of NETs-like reticulated cruciform DNA nanomachines and antibacterial mechanisms
Objective To explore the antibacterial performance and mechanism of reticulated cruciform DNA nanomachines mimicking neutrophil extracellular traps(NETs).Methods In this study,a cross-shaped DNA nanostructure was synthesized using the one-step method to form a network under the polymerization of magnesium ions.The network was used as a template for inlaying reduced Cu0 to form reticulated DNA-templated copper nanoclusters(CuNPs).The cruciform DNA formation process was characterized by polyacrylamide gel electrophoresis(PAGE).The reticulated cruciform DNA nanomachine was characterized by atomic force microscopy(AFM)and energy dispersive spectrometer(EDS).The optimal concentrations of Cu2+,Mg2+and DNA in the reticulated cruciform DNA nanomachine was determined by plate coating experiments.The antibacterial performance of the reticulated cruciform DNA nanomachine against Escherichia coli(E.coli),Staphylococcus aureus and Klebsiella pneumoniae was verified by plate coating experiments.The aggregation effect of E.coli was evaluated by crystal violet staining,and the changes on the membrane surface of E.coli were observed by scanning electron microscopy(SEM),in order to explore the antibacterial mechanism of the reticulated cruciform DNA nanomachine.Results PAGE showed that the band migration distance of the four DNA strands was the smallest after co-incubation.AFM showed that the DNA structure was reticulated and evenly distributed,and the Cu,Mg,and P elements coexisted in the structure.The optimal concentrations of Cu2+,Mg2+,and DNA required for the synthesis of the reticulated cruciform DNA nanomachine were 1 mmol/L,100 mmol/L,and 20 μmol/L,respectively.The reticulated cruciform DNA nanomachine had the ability to inhibit E.coli,Staphylococcus aureus and Klebsiella pneumoniae,with survival rates of 39.72％,43.56％and 60.57％,respectively.The reticulated cruciform DNA nanomachine had an aggregation effect on E.coli.The surface of the bacterial film exhibited shrinkage and fractures.Conclusion The reticulated cruciform DNA nanomachine constructed in this study can aggregate E.coli,leading to the shrinkage and fracturing of the bacterial film,and exhibiting antibacterial effects.
万方数据
维普
