摘要
质谱流式细胞术(CyTOF)将传统流式细胞技术与质谱分析技术相结合,其具有独特的金属标签标记、无需繁琐的补偿计算、高通量细胞分析以及多样化数据信息处理等优势,可实现单细胞水平同时检测40多个参数,其应用已涉及医学领域中的信号通路、生长发育、肿瘤微环境、自身免疫性疾病、感染、免疫治疗以及器官移植等方面.该文围绕CyTOF在儿科疾病临床诊疗与研究的最新应用进展进行阐述,以期将CyTOF更好地应用于儿科疾病的诊治.
Abstract
Mass cytometry by time of flight(CyTOF)combines traditional flow cytometry with mass spectrometry,allowing for high-throughput,multiplexed measurements of up to 40 parameters.It offers advantages such as unique metal label tags,no need for compensation calculations,high throughput,and diversified data processing.CyTOF has been applied in many fields of medicine,including signaling pathways,growth and development,tumor microenvironments,autoimmune diseases,infections,immunotherapy,and organ transplantation.This article reviews recent progress in the application of CyTOF in the clinical diagnosis,treatment,and research of pediatric diseases to enhance its utility in diagnosis and treatment.
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