Establishment of reference intervals for refined immune cell subsets by multi-parameter flow cytometry
Objective The aim of this study is to establish the reference interval of refined immune cell subsets by multi-parameter flow cytometry.Methods In this cross sectional study,a total of 326 healthy participants were included and divided into two groups based on age:18-40 years old group and 41-60 years old group.Peripheral venous blood was collected in a fasting status.Flow cytometry tests were performed according to previous consensus article.The analysis of reference interval was conducted according to the documents of Clinical and Laboratory Standards Institute(CLSI)EP28-A3c and Health Industry Standards of the People's Republic of China WS/T 402-2024.Results The T,B,NK,DC and monocyte refined immune cell subsets applicable to the reference range of the general population mainly include:CD3+(56.4％-83.3％),CD4+TEMRRA(0.2％-11.6％),CD4+TEM(14.9％-52.8％),CD4+CD28+(76.3％-99.9％),CD19+CD5+(9.7％-45.8％),CD19+CD27-(45.7％-90.0％),CD19+CD27+(9.8％-54.0％),CD3+CD16+CD56+(1.3％-20.2％),CD3-CD19-CD20-CD14-CD56-HLA-DR+(0.4％-2.0％),Monocyte Mo1 subset CD14+CD16-(46.3％-94.6％),monocyte Mo2 subset CD14+CD16+(2.8％-49.7％),etc.When the Z-value between different age groups was higher than Z*(3.50 cut-off value),the reference intervals of these subsets should be established independently according to age.Conclusions In this study,the reference intervals of refined subsets of immune cells by multi-parameter flow cytometry has been preliminarily established.For those subgroups that meet the grouping criteria,age should be fully considered in clinical applications and laboratory validation.
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维普
