Application of T-lymphocyte detailed subsets immunosurveillance in B-lymphoproliferative disorder
Objective To investigate the difference in the proportions of T-lymphocyte subsets and the immunological monitoring profiles between patients with B-lymphoproliferative disorder(B-LPD)and healthy individuals.Methods This clinical observational study involved 194 B-LPD patients[122 males,72 females,average age(64+20)]treated in Qilu Hospital of Shandong University from May 14th,2022 to January 19th,2024.The patient cohort included 76 cases of chronic lymphocytic leukemia(CLL),23 cases of diffuse large B-cell lymphoma(DLBCL),17 cases of follicular lymphoma(FL),12 cases of hair-cell leukemia(HCL),10 cases of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia(LPL/WM),26 cases of mantle cell lymphoma(MCL),20 cases of marginal zone lymphoma(MZL),and 10 cases of unclassified CD5-B lymphocytic proliferative disease unclassified(B-LPD-U).At the same time,45 health control(HC)samples[22 males,23 females,aged 62(±17)]from the medical examination center of our hospital in November 18th,2023 were collected.The T cell subsets were meticulously analyzed using flow cytometry,including CD4+naive T cell[CD4+TN(CD3+CD4+CD45RA+CD62L+)],CD4+central memory T cell[CD4+TCM(CD3+CD4+CD45RA-CD62L+)],CD4+terminally differentiated effector memory cells expressing CD45RA[CD4+TEMRA(CD3+CD4+CD45RA+CD62L-)],CD4+effector memory T cell[CD4+TEM(CD3+CD4+CD45RA-CD62L-)],CD8+TN(CD3+CD8+CD45RA+CD62L+),CD8+TCM(CD3+CD8+CD45RA-CD62L+),CD8+TEMRA(CD3+CD8+CD45RA+CD62L-),CD8+TEM(CD3+CD8+CD45RA-CD62L-),naive regulatory T cell[nTreg(CD3+CD4+CD127dim+CD25+CD45RA+)],memory Treg[mTreg(CD3+CD4+CD127dim+CD25+CD45RA-)]and activated Treg[aTreg(CD3+CD4+CD127dim+CD25+HLA-DR+)].The difference of T cell subsets data were compared by Mann-Whitney test.Result The proportionof CD3+T cells within lymphocyte in B-LPD groups(CCL group 13.15％,DLBCL group 41.75％,FL group 30.52％,HCL group 34.24％,LPL/WM group 40.58％,MCL group 20.67％,MZL group 26.36％,CD5-B-LPD-U group 17.49％)was significantly lower than HC group(64.85％).The percentage of CD4+T cells in B-LPD groups(CCL group 46.63％,DLBCL group 40.76％,FL group 42.77％,HCL group 43.81％,LPL/WM group 43.02％,MCL group 45.58％,MZL group 43.95％,CD5-B-LPD-U group 46.91％)was also significantly lower than HC group(54.61％).Conclusions B-LPD diseases impair T cell immune function.The increased presence of CD4+TEM,the reduced level of CD8+TEMRA and the elevated aTreg suggest that B-LPD may suppress CD8+T cell-mediated cytotoxicity and enhance the immunosuppressive activity of Treg.
Lymphoproliferative disordersB-lymphoproliferative disorderCD4+T cellCD8+T cellRegulatory T cellFlow cytometry
万方数据
维普
