Development and clinical application of a portable CYS-C instant detection transmission turbidimeter based on smartphones
Objective To develop a portable and easy-to-operate Cystatin C(CYS-C)transmission turbidimeter based on smartphones.Methods Leveraging the sensitivity of smartphone ambient light sensors to optical signals and the principle of immunoturbidimetry,a smartphone-based turbidimeter was designed and fabricated for point-of-care testing(POCT)of CYS-C in serum.The main structure of the smartphone turbidimeter was created using 3D printing technology and assembled according to the designed circuits and optical paths.At the same time,the stability of the light source and the feasibility of the device were verified.Finally,the sensitivity,specificity,and concordance rate of the smartphone turbidimeter were assessed by comparing its results with those from 127 serum samples of automated biochemical analyzer used in clinic.Results During device performance testing,the smartphone turbidimeter exhibited a coefficient of variation(CV)of 0.9％for light intensity over a continuous 60-minute operation.Additionally,the CV values were below 5％in batch repeat tests of CYS-C samples.The detection results of CYS-C in 127 serum samples showed a sensitivity of 84.4％,specificity of 95.8％,and concordance rate of 92.9％,with a correlation coefficient of r=0.902 9 compared to the automatic biochemistry analyzer.Conclusion The developed smartphone turbidimeter demonstrated good stability,consistency,and accuracy,with a high correlation to results from fully automated biochemical analyzers,confirming its practicality and effectiveness in clinical detection of CYS-C.
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维普
