Drug resistance genes in carbapenem-resistant Enterobacteriaceae strains isolated from a three-A hospital and their homology
OBJECTIVE To observe the drug resistance phenotypes and drug resistance genes in carbapenem-resist-ant Enterobacteriaceae strains isolated from a three-A hospital,analyze the homology and explore the causes for occurrence of drug resistance so as to provide theoretical basis for control of transmission of infection.METHODS The strains that were identified as CRE by clinical culture of specimens were collected from Zibo First Hospital from Jan 2019 to Sep 2021.The carbapenem-resistant phenotypes of the CRE strains were screened out by mCIM and eCIM tests,carbapenem-resistant genes were detected for the strains by polymerase chain reaction(PCR),the transmission capability of the drug resistance genes was observed by conjugation experiment,and the homolo-gy of the strains was analyzed by multilocus sequence typing(MLST).RESULTS Among the CRE strains,there were 54 strains of Escherichia coli and 15 strains of Klebsiella pneumoniae.Of the carbapenem-resistant E.coli strains,38 strains were positive for blaNDM-1,9 strains were positive for blaOXA-23,and 7 strains were positive for blaOXA-48 Of the carbapenem-resistant K.pneumoniae strains,3 strains were positive for blaKPC-gp,3 strains were positive for blaKPC-qc,and 9 strains were positive for blaOXA-48.The result of conjugation experiment showed that blaNDM-1 and blaKPC-gp can transmit through conjugation experiment.ST10,ST23,ST131 and ST117 were the ST types of the E.coli strains,and the clustering analysis indicated that the ST types belonged to different clone groups.ST11,ST23 and ST342 were the ST types of K.pneumoniae strains,and the clustering analysis showed that the ST types belonged to different clone groups.CONCLUSION blaNDM-1 is the major gene leading to the drug resistance of E.coli strains among the CRE strains isolated from the area,and blaOXA-48is the major gene leading to the drug resistance of K.pneumoniae strains.MLST clustering analysis shows that there are clonal transmis-sions of multiple clone complex groups among the carbapenem-resistant E.coli and K.pneumoniae strains.It is necessary for the hospital to strengthen the prevention,control and surveillance.
NETL
NSTL
万方数据
维普
