黄芪多糖对肺炎支原体感染大鼠的干预效果
Intervention effect of astragalus polysaccharide on rats with Mycoplasma pneumoniae infection
苏自强 1谢荣芳 1蔡凯晋 1吴玉塘 1陈立松 1张保根2
作者信息
- 1. 福建医科大学附属第二医院急诊科,福建泉州 362000
- 2. 福建医科大学附属第二医院中医科,福建泉州 362000
- 折叠
摘要
目的 探究黄芪多糖对肺炎支原体感染大鼠的干预效果及其对白细胞介素-6(IL-6)/磷酸化信号转导与转录激活因子3(STAT3)信号通路的影响.方法 选取43只SPF级Wistar雄性大鼠,10只作为空白组,其余33只建立肺炎支原体感染模型,最终有30只建模成功,并分为模型组、药物对照组、黄芪多糖组各10只,药物对照组、黄芪多糖组分别进行孟鲁司特钠、黄芪多糖干预,空白组、模型组进行0.9％氯化钠溶液干预,7 d后光镜下观察大鼠病理变化,采用酶联免疫吸附测定法(ELISA)检测炎性因子、氧化应激反应指标水平,实时荧光定量PCR检测IL-6、STAT3 mRNA表达量,免疫印迹法(WB)检测IL-6/STAT3信号通路相关蛋白相对表达量.结果 与空白组比较,模型组、药物对照组、黄芪多糖组肿瘤坏死因子-α(TNF-α)、C-反应蛋白(CRP)、丙二醛(MDA)水平、IL-6、STAT3 mRNA及蛋白相对表达量上升,白细胞介素-10(IL-10)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)水平下降(P＜0.05);与模型组比较,药物对照组、黄芪多糖组TNF-α、CRP、MDA、IL-6、STAT3 mRNA及蛋白相对表达量下降,IL-10、GSH-Px、SOD水平上升(P＜0.05),但黄芪多糖组TNF-α、CRP、MDA水平、IL-6、STAT3 mRNA及蛋白相对表达量高于药物对照组,IL-10、GSH-Px、SOD水平低于药物对照组(P＜0.05).结论 肺炎支原体感染大鼠经黄芪多糖干预后炎症及氧化应激反应减轻,感染症状缓解,其机制可能与IL-6/STAT3信号通路得到抑制有关.
Abstract
OBJECTIVE To explore the intervention effect of astragalus polysaccharides on the rats with Mycoplas-ma pneumoniae infection and observe the impact on interleukin-6(IL-6)/phosphorylated signal transducer and ac-tivator of transcription 3(STAT3)signaling pathway.METHODS Totally 43 male SPF Wistar rats were enrolled in the study,10 of which were assigned as the blank group,and the rest of 33 were established for models of M.pneumoniae infection,30 rats were modeled successfully and were divided into the model group,the drug con-trol group and the astragalus polysaccharide group,with 10 rats in each group.The drug control group and the as-tragalus polysaccharide group were treated with montelukast sodium and astragalus polysaccharide,respectively;the blank group and the model group were treated with 0.9％sodium chloride solution.The pathological changes of the rats were observed by light microscope after the treatments for 7 days.The levels of inflammatory factors and oxidative stress indexes were detected by enzyme-linked immunosorbent assay(ELISA);the expression levels of IL-6 and STAT3 mRNA were detected by means of real-time fluorescent quantitative PCR;the relative expres-sion levels of IL-6/STAT3 signaling pathway-related proteins were detected by Western Blot(WB).RESULTS As compared with the blank group,the levels of tumor necrosis factor-α(TNF-α),C-reactive protein(CRP),ma-londialdehyde(MDA)and relative expression levels of IL-6 and STAT3 mRNA and proteins of the model group,the drug control group and the astragalus polysaccharide group were elevated,while the levels of interleukin-10(IL-10),glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)declined(P＜0.05).The relative expression levels of TNF-α,CRP,MDA,IL-6,STAT3 mRNA and proteins of the drug control group and the as-tragalus polysaccharide group were lower than those of the model group,while the levels of IL-10,GSH-Px and SOD of the drug control group and the astragalus polysaccharide group were higher than those of the model group(P＜0.05).The levels of TNF-α,CRP and MDA and relative expression levels of IL-6,STAT3 mRNA and pro-teins were higher in the astragalus polysaccharide group then in the drug control group,while the levels of IL-10,GSH-Px and SOD of the astragalus polysaccharide group were lower than those of the drug control group(P＜0.05).CONCLUSION The astragalus polysaccharide may alleviate the inflammatory and oxidative stress response of the rats with M.pneumoniae infection and relieve the symptom of infection.The mechanism may be associated with the inhibition of IL-6/STAT3 signaling pathways.
关键词
肺炎支原体感染/大鼠/黄芪多糖/干预/白细胞介素-6/磷酸化信号转导与转录激活因子3信号通路Key words
Mycoplasma pneumoniae infection/Rat/Astragalus polysaccharide/Intervention/Interleukin-6/phosphorylated signal transducer and activator of transcription 3 signaling pathway引用本文复制引用
基金项目
福建省科学技术厅创新战略研究项目(2020R0170)
出版年
2024
NETL
NSTL
维普
万方数据