Anti-inflammatory effect of Houttuynia extract on rats with Mycoplasma pneumoniae infection and mechanisms of action
OBJECTIVE To observe the anti-inflammatory effect of Houttuynia extract on rats with Mycoplasma pneumoniae infection and analyze the mechanisms of action.METHODS The Wistar rats of SPF grade were divid-ed into the blank group,the M.pneumoniae infection model group,the low-dose houttuynia extract group and the high-dose houttuynia extract group,with 9 rats in each group.The blank group and the model group were only treated with normal saline for gavage,the low-dose group and the high-dose group were respectively treated with 125 mg/kg and 500 mg/kg of houttuynia extract for gavage.The levels of peripheral blood transforming growth factor-β(TGF-β),Krebs Von den Lungen-6(KL-6),surface active protein D(SP-D),inflammatory factors and the relative expression levels of Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88)and nu-clear factor κB(NF-κB)mRNA were detected after the treatments for 7 consecutive days;the expression levels of TLR4,MyD88 and NF-κB proteins in lung tissues were detected by Western Blot.RESULTS The levels of TGF-β,KL-6,SP-D,IL-13,TNF-α,the expression levels of TLR4,MyD88,NF-κB mRNA,and the expression lev-els of TLR4,MyD88 and NF-κB proteins were higher in the model group,the low-dose houttuynia extract group and the high-dose houttuynia extract group than in the blank group,while the IL-12 level was lower in the model group,the low-dose houttuynia extract group and the high-dose houttuynia extract group than in the blank group(P＜0.05).The levels of TGF-β,KL-6,SP-D,IL-13,TNF-α,the expression levels of TLR4,MyD88,NF-κB mRNA,and the expression levels of TLR4,MyD88 and NF-κB proteins were lower in the high-dose houttuynia extract group than in the model group and the low-dose houttuynia extract group;the IL-12 level of the high-dose houttuynia extract group was higher than that of the model group and the low-dose houttuynia extract group(P＜0.05).CONCLUSION As a treatment intervention,Houttuynia extract may alleviate the local inflammatory response or even the inflammatory responses in bronchia and lung tissues so as to achieve favorable intervention effect.
Houttuynia extractMycoplasma pneumoniae infectionAnti-inflammatory effectMechanism of action
NETL
NSTL
万方数据
维普
