Lifetime of multidrug-resistant organisms adhered to surfaces of needle-free infusion connectors and effect of disinfection
OBJECTIVE To explore the lifetime of multidrug-resistant organisms(MDROs)adhered to surfaces of needle-free infusion connectors and observe the effects of disinfectants on disinfection of the MDROs.METHODS In strict accordance with aseptic operation regulations,four types of MDROs were evenly smeared on 5 types of commonly used needle-free infusion connectors,respectively(type A,B,C,D and E).The samples were col-lected for culture of viable counts after the attachment for 24 hours,48 hours,72 hours,1 week,2 weeks,3 weeks and 4 weeks.The survival status of the MDROs on the connector surfaces was monitored.The effects of 75％alcohol,0.5％iodophor and 2％chlorhexidine on disinfection of the MDROs that adhered to the surfaces of needle-free infusion connectors were evaluated.RESULTS The experiment showed that the colony counts of methi-cillin-resistant Staphylococcus aureus(MRSA)strains that adhered to the surfaces of type A,B,C and D needle-free infusion connectors were relatively great when temperature was 35℃,and the survival time exceeded 4 weeks;the survival time of carbapenem-resistant Klebsiella pneumoniae(CRKP)that adhered to the surfaces of type A needle-free infusion connectors was the longest and exceeds 4 weeks;the survival time of carbapenem-re-sistant Acinetobacter baumannii(CRAB)that adhered to the surfaces of type C needle-free infusion connectors was longest and exceeded 4 weeks.The MDROs that adhered to the surfaces of type E needle-free infusion connec-tors did not survive after the attachment for 4 weeks.All of the three types of disinfectants could achieve killing effect on the four types of MDROs when they acted on the surfaces of the infusion connectors for 5 and 10 min,and the killing effect was even better when the acting time was 10min;the killing effect on the MDROs that ad-hered to the type E needle-free infusion connectors was better than that on the MDROs that adhered to other four types of connectors.CONCLUSION The reasonable design of connectors may reduce the colonized and residual MDROs and improve the disinfection effect.It is necessary to choose the infusion connectors with smooth sur-faces,good airtightness,high perspectivity and texture adverse to growth of pathogens and effectively disinfect in strict accordance with the disinfection regulations so as to reduce the incidence of MDROs infection due to the infu-sion connectors.
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万方数据
维普
